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目的了解福建省耐多药结核分枝杆菌分子流行病学特征,为控制耐多药肺结核提供参考依据。方法采用多位点数目可变串联重复序列基因分型(MLVA)方法,对30个监测点纳入监测的所有耐多药结核分枝杆菌分离菌株DNA进行检测,使用BioNumerics(Version 4.5)软件进行聚类分析。结果 76株耐多药结核分枝杆菌被分为Ⅰ、Ⅱ、Ⅲ三大基因群,分别包含Ⅰ群5株(6.6%)、Ⅱ群68株(89.5%)、Ⅲ群3株(3.9%);在株水平基因分型上,有19株菌成7簇,各包含2～4株菌,成簇菌株来源于同一县区或不同县区。结论福建省耐多药结核分枝杆菌菌株主要流行株为Ⅱ群菌株;部分菌株存在县区内,甚至跨县域的近期传播流行。
Objective To understand the molecular epidemiological characteristics of multidrug-resistant Mycobacterium tuberculosis in Fujian province and provide a reference for the control of multidrug-resistant pulmonary tuberculosis. Methods The MLVA method was used to detect the DNA of all M. multidrug-resistant Mycobacterium strains that were monitored in 30 monitoring sites. BioNumerics (Version 4.5) software was used for poly Class analysis Results 76 Mycobacterium multidrug-resistant strains were divided into three major gene groups Ⅰ, Ⅱ and Ⅲ, including 5 strains of group Ⅰ (6.6%), 68 strains of group Ⅱ (89.5%), 3 strains of group Ⅲ (3.9% ). In the genotyping of strains, 19 strains were clustered into 7 clusters, each containing 2 ~ 4 strains. The cluster strains originated from the same county or different counties. Conclusions The main epidemic strains of multidrug-resistant Mycobacterium tuberculosis strains in Fujian Province are Group Ⅱ strains. Some of the strains exist in recent years in counties and even in counties.