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目的：研究抗Ｆａｓ抗体对人肾小球系膜细胞的致凋亡作用，探讨ＦａｓＦａｓＬ在肾脏损伤中的作用。方法：常规方法分离、培养人肾小球系膜细胞（Ｍｃｓ），并传代鉴定，用不同浓度的抗Ｆａｓ抗体（０，２，５，１０μｇ／ｍｌ）刺激４～６代Ｍｃｓ１６ｈ后，荧光染色观察Ｍｃｓ凋亡变化，二苯胺法和ＤＮＡ凝胶电泳方法定量和定性分析ＭｃｓＤＮＡ片段化变化。结果：抗Ｆａｓ抗体可诱导Ｍｃｓ凋亡，且致凋亡效应呈依赖性关系。结论：ＦａｓＦａｓＬ系统在Ｍｃｓ凋亡中起着重要作用。
Objective: To study the anti-Fas antibody induced apoptosis in human mesangial cells, and to explore the role of Fas-FasL in renal injury. Methods: Human mesangial cells (Mcs) were isolated and cultured by routine methods and identified by passage. Fluorescent staining was performed after 4-6 generations of Mcs16h stimulation with different concentrations of anti-Fas antibody (0, 2, 5 and 10μg / ml) Apoptosis of Mcs was observed. Diphenylamine and DNA gel electrophoresis were used to quantitatively and qualitatively analyze the fragmentation of McsDNA. Results: Anti-Fas antibody can induce apoptosis of Mcs and induce apoptosis-dependent. Conclusion: Fas-FasL system plays an important role in the apoptosis of Mcs.