目的:通过比较马钱属3种药材山马钱、马钱子、吕宋果中士的宁和马钱子碱的含量,探讨山马钱与吕宋果是否可以作为马钱子的替代资源。方法:采取HPLC法,条件:Agilent C18柱,流动相为水-冰醋酸-三乙胺(230∶2.4∶0.3)(A)∶甲醇(B)为75∶25,检测波长254 nm,流速1 mL.min-1,柱温30℃,进样体积10μL。结果:士的宁和马钱子碱的质量浓度分别在0.224～2.261 mg.mL-1(r=0.999 4,n=5)和0.124～1.241 mg.mL-1(r=0.999 8,n=5)范围内呈良好的线性关系,平均回收率分别为99.01%和98.91%;吕宋果中士的宁含量在3种药材中最高,其马钱子碱的含量低于马钱子,而山马钱中的士的宁和马钱子碱的含量均较低。结论:该法精确,简便,重复性好;吕宋果有可能成为马钱子的替代资源,而山马钱不适宜作为其药用资源。 OBJECTIVE: To compare the content of strychnine and strychnine in Strychnos, Strychnos, Luzon scholar strychnos, and explore whether the Sima Qian and Luzon fruit can be used as substitutes for Strychnos. Methods: The HPLC method was used under the conditions of Agilent C18 column with the mobile phase of water - glacial acetic acid - triethylamine (230: 2.4: 0.3) (A): methanol mL.min-1, column temperature 30 ℃, injection volume 10μL. Results: The concentrations of strychnine and brucine were 0.224 ~ 2.261 mg.mL-1 (r = 0.999 4, n = 5) and 0.124-1.241 mg.mL-1 (r = 0.999 8, n = 5), the average recoveries were 99.01% and 98.91%, respectively. The content of strychnine in Luzon was the highest among the three herbs, the content of brucine was lower than that of Strychnos, Taxi strychnine and brucine content are low. Conclusion: The method is accurate, simple and reproducible. Luzon fruit may become an alternative resource of Strychni, while Sima Qian is not suitable as its medicinal resources.