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目的 :为检测HBV基因免疫BALB/c小鼠的CTL应答提供靶细胞。方法 :将已构建的含HBV膜蛋白L基因的质粒pcDNA3 HBVL以电穿孔法转染P815细胞。阳性克隆通过抗G4 18抗性进行筛选 ,以已转染 pcDNA3的P815细胞系作为对照 ,用免疫细胞化学染色法进一步鉴定。结果 :通过第 1轮筛选 ,获得 6株抗G4 18抗性P815细胞克隆 ,其中 1株稳定表达HBVL蛋白。结论 :建立了可稳定表达HBVL蛋白的P815细胞系
OBJECTIVE: To provide target cells for the detection of CTL responses in BALB / c mice immunized with HBV. Methods: The plasmid pcDNA3 HBVL containing HBV membrane protein L gene was transfected into P815 cells by electroporation. Positive clones were screened by anti-G4 18 resistance, and the P815 cell line transfected with pcDNA3 was used as a control and further identified by immunocytochemical staining. Results: Six anti-G4 18 resistant P815 cell clones were obtained by the first round of screening, of which one strain stably expressed HBVL protein. Conclusion: The P815 cell line that can stably express HBVL protein was established