论文部分内容阅读
本文应用免疫荧光法,以T细胞亚群单克隆抗体,研究婴幼儿呼吸道合胞病毒(RSV)毛细支气管炎急性期及感染后反覆喘鸣儿的T抑制细胞量的变化。急性期CD_3、CD_6、CD_8,细胞均明显减少,出院时CD_3,细胞明显增多,CD_4细胞呈升高趋势,CD_8细胞变化极少。感染后反覆喘鸣儿CD_8细胞亦呈低水平状态,与正常儿比较,均呈显著性差异(p<0.01)。急性期RSV毛细支气管炎患儿与不伴喘鸣的RSV阴性患儿相比前者CD_3、CD_8量少,但不呈统计学差异(p>0.05)。实验提示T细胞免疫反应尤其是CD_8细胞参与了RSV毛细支气管炎喘鸣的发病与转归。
In this paper, immunofluorescence method to T lymphocyte subsets of monoclonal antibodies to study infants and young children respiratory syncytial virus (RSV) bronchitis in acute phase and repeated wheezing after infection T-suppressor cell volume changes. Acute phase of CD_3, CD_6, CD_8, cells were significantly reduced, discharged CD_3, significantly increased cells, CD_4 cells showed an upward trend, CD_8 cells rarely change. CD_8 cells were also found to be in a low level after repeated episodes of wheezing, which were significantly different from those of normal children (p <0.01). The children with acute RSV bronchiolitis had less CD_3 and CD_8, but no statistical difference (P> 0.05) compared with those without RSV. Experimental results suggest that T cell immune response, especially CD8 cells, is involved in the pathogenesis and outcome of RSV bronchiolitis.