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目的观察胃癌组织XAB2基因及蛋白表达的变化,探讨其与胃癌发生发展的可能关系。方法选取34例胃癌患者的肿瘤组织及癌旁正常组织,采用real-time RT-PCR技术检测XAB2 mRNA的表达;采用免疫组化方法检测胃癌组织、癌旁正常组织、胃炎组织XAB2蛋白的表达;分析不同临床病理指标下胃癌组织XAB2蛋白的表达差异。结果胃癌组织XAB2 mRNA表达显著低于癌旁正常组织(P<0.01),且在胃癌进展早期(Ⅰ+Ⅱ期)即明显下降。免疫组化检测显示:胃癌组织中XAB2蛋白表达的阳性率为50.0%(17/34),明显低于配对癌旁正常组织82.4%(28/34)和胃炎组织91.1%(31/34),差异具有统计学意义(P<0.01),而后二者间无统计学差异。胃癌组织XAB2蛋白表达与胃癌分化程度正相关(P=0.041),与肿瘤浸润深度、肿瘤大小负相关(P=0.019、0.027);在不同性别、年龄、肿瘤部位、有无淋巴结转移、TNM分期间无统计学差异。结论XAB2低表达可能与胃癌发生、进展密切相关,值得深入研究以利于临床胃癌诊治。
Objective To observe the changes of XAB2 gene and protein expression in gastric cancer and to explore its possible relationship with the occurrence and development of gastric cancer. Methods The tumor tissues and adjacent normal tissues of 34 gastric cancer patients were selected. The expression of XAB2 mRNA was detected by real-time RT-PCR. The expression of XAB2 protein was detected by immunohistochemistry in gastric cancer tissues, adjacent normal tissues and gastritis tissues. Analysis of different clinicopathological parameters of gastric cancer XAB2 protein expression differences. Results The expression of XAB2 mRNA in gastric cancer tissues was significantly lower than that in adjacent normal tissues (P <0.01), and significantly decreased in the early stages of gastric cancer (stage Ⅰ + Ⅱ). Immunohistochemistry showed that the positive rate of XAB2 protein expression was 50.0% (17/34) in gastric cancer tissues, which was significantly lower than that in matched normal tissues (82.4%) and gastric cancer tissues (91.1%, 31/34) The differences were statistically significant (P <0.01), but there was no significant difference between them. There was a positive correlation between the expression of XAB2 protein and the degree of gastric cancer differentiation (P = 0.041), but negatively correlated with the depth of tumor invasion and tumor size (P = 0.019,0.027). There was no difference in lymph node metastasis, lymph node metastasis, There was no significant difference between the two groups. Conclusion The low expression of XAB2 may be closely related to the occurrence and progress of gastric cancer. It is worth further study to facilitate the diagnosis and treatment of clinical gastric cancer.