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目的 探讨γ 干扰素(IFN- γ)、转化生长因子β3(TGFβ3) 对体外培养的创面成纤维细胞生物学作用的影响。方法 采用体外细胞培养技术,通过 MTT测定法、3 H TdR 掺入法、成纤维细胞胶原凝胶模型研究 IFN- γ、TGFβ3对不同时期创面来源的成纤维细胞的增殖、DNA合成及其在胶原凝胶中收缩的变化。结果 IFN -γ和TGFβ3 在体外对正常皮肤真皮和烧伤后不同时期创面来源的成纤维细胞的生物学作用不同: (1)相同浓度的两种因子均能明显抑制正常真皮和伤后10、14 d创面成纤维细胞,对伤后28、35 d的创面成纤维细胞γ 干扰素的抑制作用较弱、TGFβ3 作用则不明显;(2)对成纤维细胞在胶原凝胶中的收缩能力,两种因子均有明显的抑制作用,而最强的作用均表现在对伤后21 d的创面成纤维细胞上。结论 γ -干扰素和转化生长因子β3 可能参与了调节创面成纤维细胞的表型转化与调控而影响其生物学特征,进一步的研究有利于为临床用生物学方法在创面愈合过程中控制瘢痕增生或挛缩提供参考依据。
Objective To investigate the effects of IFN-γ and TGFβ3 on the biological effects of wound fibroblasts cultured in vitro. Methods MTT assay, 3 H TdR incorporation method and fibroblast collagen gel model were used to study the effects of IFN-γ and TGFβ3 on proliferation, DNA synthesis and DNA synthesis of wound-derived fibroblasts at different stages in vitro Shrinkage changes in the gel. Results The biological effects of IFN-γ and TGFβ3 on wound-derived fibroblasts in normal dermis and burn wounds in vitro were different in vitro: (1) Both of the two factors at the same concentration significantly inhibited normal dermis and wounds 10, 14 d wound fibroblasts, the wound fibroblasts on the 28,35 d injury γ interferon inhibition is weak, the effect of TGFβ3 is not obvious; (2) the ability of fibroblasts in collagen gel contraction, two All kinds of factors have a significant inhibitory effect, and the strongest effect is manifested in the wound on the 21st day wound fibroblasts. Conclusions γ - interferon and transforming growth factor - β3 may be involved in the regulation of phenotypes of wound fibroblasts and their biological characteristics, and further studies are helpful to control the scar hyperplasia in clinical wound healing process Or contracture to provide a reference.