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目的 研究人乳腺癌异种移植的自发转移、遗传稳定性和微转移的检测方法。方法 将人乳腺癌新鲜完整组织移植于裸鼠并连续传代 ,移植瘤细胞体外培养、再移植裸鼠并在 3个微卫星位点分析人乳腺癌、裸鼠移植瘤及其转移灶的微卫星DNA。结果 原位移植的成瘤率为 88.6 % (31/35 )、转移率为 41.9% (13/ 31)。移植瘤细胞体外培养成功 ,其再移植裸鼠成瘤率和转移率均达 10 0 %。裸鼠移植瘤及其转移灶在 3个位点与人乳腺癌具有相同的微卫星DNA。结论 完整组织原位移植法可提高人乳腺癌异种移植的成功率和转移率。异种移植瘤及其传代肿瘤保持了遗传稳定性。
Objective To study the spontaneous metastasis, genetic stability and micrometastasis detection methods in human breast cancer xenografts. METHODS: Human intact fresh breast cancer tissue was transplanted into nude mice and serially passaged. The transplanted tumor cells were cultured in vitro, nude mice were transplanted, and microsatellites of human breast cancer, nude mice transplanted tumors and their metastases were analyzed at 3 microsatellite loci. DNA. Results The tumorigenicity of orthotopic transplantation was 88.6 % (31/35) and the metastatic rate was 41.9% (13/31). Tumor cells were successfully cultured in vitro, and the tumor-bearing rate and metastasis rate of retransplanted nude mice reached 100%. The nude mice transplanted tumors and their metastatic foci have the same microsatellite DNA at three sites as human breast cancer. Conclusion Intact tissue orthotopic transplantation can improve the success rate and metastasis rate of human breast cancer xenografts. Xenograft tumors and their passaged tumors maintained genetic stability.