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目的探索亚甲基四氢叶酸还原酶基因多态性与原发性高血压的关联性关系。方法 2015年4月在辽宁省西北部高血压高发区汉族人群中收集原发性高血压患者和健康对照者共计1065人(原发性高血压患者547例,健康对照者518人)的流行病学资料和血样供检测,应用Sequenom基质辅助激光解吸电离飞行时间质谱的进行SNPs分型。应用非条件Logistic回归和分层分析进行统计学分析。结果 MTHFR677C/T和MTHFR-1622A/G位点的等位基因型频率在高血压组和正常对照组两组间分布均没有显著性差异(χ~2=0.738,P>0.05;χ~2=4.437,P>0.05);两个多态位点的等位基因频率在高血压组和正常对照组之间也无统计学显著性差异(χ~2=0.059,P>0.05;χ~2=1.243,P>0.05)。按照年龄和体重指数进行分层分析后,在40~年龄组和肥胖组中,MTHFR-1622A/G位点与高血压存在显著性关联(P<0.05)。结论在辽宁省西部高血压高发区汉族人群中MTHFR677C/T和MTHFR-1622A/G位点可能不是高血压的易感基因。
Objective To explore the association between methylenetetrahydrofolate reductase gene polymorphism and essential hypertension. Methods A total of 1065 patients (547 patients with essential hypertension and 518 patients with healthy controls) were enrolled in this study from January to April 2015 in Han nationality with high essential hypertension in northwest Liaoning province. Materials and blood samples were tested for SNPs typing using Sequenom matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Unconditional logistic regression and stratified analysis were used for statistical analysis. Results There was no significant difference in allele frequencies between MTHFR677C / T and MTHFR-1622A / G loci in the hypertensive group and normal control group (χ ~ 2 = 0.738, P> 0.05; χ ~ 2 = 4.437, P> 0.05). There was no significant difference in allele frequency between the two polymorphic loci in hypertension group and normal control group (χ ~ 2 = 0.059, P> 0.05; 1.243, P> 0.05). After stratified analysis according to age and body mass index, MTHFR-1622A / G locus was significantly associated with hypertension in 40 ~ age group and obesity group (P <0.05). Conclusions MTHFR677C / T and MTHFR-1622A / G loci may not be the predisposing genes for hypertension in Han population of high blood pressure in western Liaoning Province.