论文部分内容阅读
目的:评价输血传播性病毒(TTV)是否具有母婴垂直传播的可能性。方法:设计2对特异性引物构成巢式PCR方法,检测了22例婴儿肝炎综合征(年龄中位数2.5月)及其16例母亲血清中TTV DNA、PCR产物克隆并测序分析。结果:在22例婴儿肝炎综合征中,仅1例TTV DNA阳性,16例母亲中5例TTV DNA阳性,检出率显著高于前者(4.5％,11％,P<0.01);唯一1例TTV DNA阳性患儿的母亲却为阴性,且患儿2个月前有血制品输入史;5例TTV DNA阳性母亲的患儿无1例阳性。结论:婴儿TTV感染率显著低于母亲,TTV通过母婴垂直传播的可能性不大。
PURPOSE: To evaluate whether transfusion transmitted virus (TTV) possesses the possibility of vertical transmission between mother and baby. Methods: Two pairs of specific primers were designed to form nested PCR. TTV DNA of 22 infants with hepatitis syndrome (median age 2.5 months) and 16 mothers’ serum were detected. The PCR products were cloned and sequenced. Results: TTV DNA was positive in only one case from 22 cases of infantile hepatitis syndrome. The positive rate of TTV DNA in 5 out of 16 mothers was significantly higher than that in the former (4.5%, 11%, P <0.01) The mother of TTV DNA positive children was negative, and the history of imported blood products was 2 months old. No positive result was found in 5 cases of TTV DNA positive mothers. Conclusion: The prevalence of TTV infection in infants is significantly lower than that of mothers. TTV is unlikely to spread vertically through mother and infant.