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目的 :建立一种适应性强、灵敏、准确的方法用于测定槐米中芦丁的含量。方法 :以 95 %乙醇为溶剂进行索氏提取 ;用醋酸乙酯 -甲酸 -水 (4∶ 1∶ 1)为展开剂分离 ;洗脱后以 10 % Al Cl3 甲醇溶液作为反应试剂 ,在 Ex=42 0 nm,Em=5 13 nm处测定荧光强度。结果 :此方法的线性范围为 0 .2～ 1.2μg/ml,平均回收率为 98.7% ,变异系数 RSD为 2 .71%。结论 :此方法稳定 ,重现性好 ,灵敏度高 ,可用于槐米的质量评价
Objective : To establish an adaptable, sensitive and accurate method for the determination of rutin in glutinous rice. METHODS: Soxhlet extraction was performed using 95% ethanol as the solvent; Separation with ethyl acetate-formic acid-water (4:1:1) as the developing agent; elution with 10% AlCl3 in methanol as the reaction reagent at Ex= The fluorescence intensity was measured at 42 nm and Em=5 13 nm. Results: The linear range of this method was 0.2-1.2μg/ml, the average recovery was 98.7%, RSD was 2.71%. Conclusion: This method is stable, with good reproducibility and high sensitivity, and can be used to evaluate the quality of japonica rice.