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目的 探讨磷脂酰肌醇-3激酶(phosphatidylinositol 3 kinase,PI3K)抑制剂LY-294002对心梗大鼠心肌肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)表达的影响。方法 45只SD大鼠,随机分为假手术组、心肌梗死组和PI3K抑制剂组。分别用Western blot和RT-PCR方法检测各组大鼠梗死区及其周围心肌TNF-α蛋白和TNF-α mRNA表达变化。结果 心肌梗死组心肌梗死区及其周围心肌TNF-α蛋白、TNF-α mRNA表达水平显著高于假手术组(p<0.05),PI3K抑制剂组心肌梗死区及其周围心肌TNF-α蛋白、TNF-α mRNA表达水平则明显低于心肌梗死组(p<0.05)。结论 PI3K能够上调心肌梗死大鼠TNF-α的表达。
Objective To investigate the effect of phosphatidylinositol 3 kinase (PI3K) inhibitor LY-294002 on the expression of tumor necrosis factor-α (TNF-α) in myocardial infarction rats. Methods 45 SD rats were randomly divided into sham operation group, myocardial infarction group and PI3K inhibitor group. Western blot and RT-PCR were used to detect the mRNA expression of TNF-α and TNF-α in infarct area and surrounding myocardium of rats in each group. Results The levels of TNF-α and TNF-α mRNA in myocardial infarction and myocardial infarction area were significantly higher than those in sham-operated group (P <0.05), but not in PI3K inhibitor group. TNF-α mRNA expression was significantly lower than myocardial infarction group (p <0.05). Conclusion PI3K can up-regulate the expression of TNF-α in rats with myocardial infarction.