论文部分内容阅读
目的:比较UV分光光度法和HPLC法测定小柴胡片体外溶出度的差异。方法:按2005版中国药典进行,以0.1MHCl为释放介质进行小柴胡片体外的溶出度试验,用紫外(UV)分光光度法在272nm处测定释放液的UV吸收度,同时使用HPLC法测定释放液的黄芩苷,利用配对t检验比较这两个方法的累积溶出度和溶出参数。结果:与HPLC法相比,UV分光光度法的累积溶出度平均偏低2.6个百分点(P<0.05),t50平均偏高1.0min(P<0.05),t90平均偏高6.5min(P<0.05)。结论:HPLC法和UV分光光度法的结果基本一致,都可以用于测定小柴胡片的体外溶出度。
Objective: To compare the in vitro dissolution of Xiaochaihu tablets by UV spectrophotometry and HPLC. METHODS: According to the 2005 edition of the Chinese Pharmacopoeia, the dissolution rate of Xiaochaihu tablets was measured using 0.1M HCl as the release medium. The UV absorbance of the release solution was measured at 272 nm using ultraviolet (UV) spectrophotometry, and the release was measured by HPLC. The liquid baicalin was used to compare the cumulative dissolution and dissolution parameters of the two methods using a paired t-test. Results: Compared with HPLC method, the cumulative dissolution rate of UV spectrophotometry was 2.6 percentage points lower (P<0.05), the average t50 was higher 1.0 min (P<0.05), and the average t90 was higher 6.5 min (P<0.05). . Conclusion: The results of HPLC and UV spectrophotometry are basically the same, and can be used to determine the dissolution rate of Xiaochaihu tablets in vitro.