论文部分内容阅读
为明确外源茉莉酸(JA)处理菜豆对美洲斑潜蝇抗性的诱导作用,采用生物测定和生化分析方法,研究了4种浓度JA喷施菜豆幼苗后,菜豆对美洲斑潜蝇的抗性及菜豆叶片过氧化物酶(POD)、多酚氧化酶(PPO)和脂氧合酶(LOX)活性的变化。结果表明,JA处理可以提高菜豆对美洲斑潜蝇的抗性及菜豆叶片3种防御酶的活性,且其抗性和防御酶活性提高程度均与JA浓度有关。0.1、1 mmol/L JA可显著降低美洲斑潜蝇成虫对菜豆的取食和产卵选择性,使卵或幼虫发育历期延长、幼虫取食量降低、蛹重减轻,而0.001和0.01 mmol/L JA仅对幼虫发育历期有显著影响。0.1 mmol/L JA处理后,菜豆叶片POD、PPO和LOX活性均显著升高,分别较对照增加33%、39%和80%,而0.001 mmol/L JA对菜豆叶片防御酶诱导作用不明显。
In order to clarify the induction effect of JA-treated beans on the resistance of leafminers, the biochemical and biochemical methods were used to study the effects of JA-treated beans on the resistance of the leaf miner Changes of peroxidase (POD), polyphenol oxidase (PPO) and lipoxygenase (LOX) activities in leaves and leaves of Cabernet Sauvignon. The results showed that JA could increase the resistance of L. chelsia to M. lividana and the activity of three kinds of defense enzymes in N. fabri, and the increase of its resistance and defense enzyme activity was related to the concentration of JA. 0.1 and 1 mmol / L JA could significantly reduce the feeding and ovipositional selectivity of N. lianensis to N. bean, prolong the developmental duration of eggs or larvae, reduce the feeding amount of larvae and reduce the pupal weight, while 0.001 and 0.01 mmol / L JA has a significant effect on larval developmental duration only. After 0.1 mmol / L JA treatment, the activities of POD, PPO and LOX increased significantly by 33%, 39% and 80% respectively compared with the control, while the induction of defense enzymes by JA at 0.001 mmol / L was not obvious.