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目的探讨γ-氨酪酸对新生大鼠缺氧缺血性脑损伤(HIBD)的治疗作用。方法采用Rice法制备新生大鼠HIBD模型,用γ-氨酪酸(GABA)灌服HIBD大鼠,用TUNEL法监测大鼠脑组织切片各时间点的细胞凋亡数,用化学方法检测各时间点血清中的超氧化物歧化酶(SOD),一氧化氮(NO),诱生型一氧化氮合酶(iNOS)的浓度。结果(1)缺氧缺血(HI)组各时间点的细胞凋亡率均明显高于假手术对照组;72h时间点的细胞凋亡率最高。(2)GABA治疗组与HI组相比,各时间点的凋亡率均明显降低、SOD值明显升高。(3)发病早期6h时间点HI、GABA两组与正常对照(N)组NO、iNOS浓度比较,差异无统计学意义。两组其余各时间点的NO、iNOS值均明显高于假手术正常对照组,HI、GABA两组比较,GABA各时间点的值均明显低于HI组。结论应用γ-氨酪酸治疗HIBD大鼠,可明显降低HIBD新生大鼠的脑细胞凋亡率。
Objective To investigate the therapeutic effect of γ-aminobutyric acid on hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods The HIBD model of neonatal rats was established by the method of Rice. HIBD rats were fed with γ-aminobutyric acid (GABA). The apoptosis of rats was observed by TUNEL method at different time points. Serum superoxide dismutase (SOD), nitric oxide (NO), and inducible nitric oxide synthase (iNOS) concentrations. Results (1) The apoptotic rate of hypoxic-ischemic (HI) group at each time point was significantly higher than that of sham-operated control group; the apoptosis rate was the highest at 72h. (2) Compared with HI group, the apoptosis rate of GABA treatment group at each time point was significantly lower and SOD value was significantly higher. (3) There was no significant difference in the concentrations of NO and iNOS between HI and GABA groups and normal control (N) group at 6h after onset. The NO and iNOS values in the rest of the two groups were significantly higher than those in sham-operated normal control group. The values of GABA in HI and GABA groups were significantly lower than those in HI group at each time point. Conclusion Treatment of HIBD rats with γ-aminobutyric acid can significantly reduce the apoptosis rate of neonatal rats with HIBD.