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目的探讨番茄红素对持续酒精摄入所致的酒精性肝病的保护作用。方法 100只健康成年SPF级昆明雄性小鼠随机分为对照组、模型组和低、中、高剂量番茄红素5组,每组20只。低、中、高剂量番茄红素组小鼠经口灌胃浓度分别为0.33g/L、0.67g/L和2.00g/L的番茄红素玉米油混悬液,对照组与模型组灌胃等体积的玉米油,灌胃容量为0.2m L/10g;3h后,对照组予等容生理盐水灌胃,其余4组予56度白酒0.12m L/10g灌胃,连续8d。末次灌胃后,禁食16h,测血清中甘油三酯(TG)、蛋白质羰基,肝匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)含量,同时计算死亡率、累积存活时间。结果与对照组相比,模型组血清TG含量、蛋白质羰基含量、肝匀浆MDA明显增加(P<0.01);与模型组相比,中、高剂量番茄红素组小鼠死亡率降低(χ2=4.800,P=0.028),存活时间延长。同时,肝匀浆SOD、GSH-Px含量均显著性高于模型组(P<0.01),而血清TG、蛋白质羰基含量,肝匀浆MDA却明显降低(P<0.05,P<0.01)。随着番茄红素灌胃剂量升高,小鼠血清TG、蛋白质羧基含量,肝匀浆MDA呈下降趋势,而SOD、GSH-Px含量呈上升趋势。结论番茄红素对小鼠酒精性肝病具有一定的拮抗作用。
Objective To investigate the protective effect of lycopene on alcoholic liver disease caused by continuous alcohol intake. Methods 100 healthy adult Kunming SPF Kunming mice were randomly divided into control group, model group and low, medium and high doses of lycopene 5 groups of 20 rats. The low, medium and high dose lycopene mice were orally fed with lycopene corn oil suspension of 0.33g / L, 0.67g / L and 2.00g / L respectively. The control group and model group were given gavage Equal volume of corn oil, gavage capacity of 0.2m L / 10g; 3h after the control group was given equal volume of saline, the other four groups of 56 degrees white wine 0.12m L / 10g gavage for 8 days. After the last gavage, fasted for 16 hours. Serum levels of triglyceride (TG), protein carbonyl, malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) content, while calculating the mortality rate, cumulative survival time. Results Compared with the control group, the levels of serum TG, protein carbonyl and liver homogenate MDA in the model group were significantly increased (P <0.01). Compared with the model group, the mortality in the medium and high dose lycopene group mice was lower (χ2 = 4.800, P = 0.028), survival time prolonged. Meanwhile, the levels of SOD and GSH-Px in liver homogenate were significantly higher than those in model group (P <0.01), while the levels of serum TG, protein carbonyl content and liver homogenate MDA were significantly decreased (P <0.05, P <0.01). With the increase of intragastric dose of lycopene, serum TG, protein carboxyl content, liver homogenate MDA decreased, while SOD, GSH-Px increased. Conclusion Lycopene has antagonistic effect on alcoholic liver disease in mice.