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目的比较外伤性和非外伤性癫痫灶组织的基因表达谱,探索外伤性癫痫的发病机制,为相关鉴定提供依据。方法收集外伤性和非外伤性癫痫灶组织样本各15例。分别提取总RNA,经逆转录标记不同的探针与人类cDNA表达谱芯片杂交,获得扫描图像,分析信号的强度和比值,对实验数据按照RNA微阵列分析法标准化,对差异表达基因进行聚类分析。结果两组样本中共有超过2 990条基因明显表达(表达超过背景2～5倍以上)。以30%样本中的表达调节水平大于1.5倍作为差异基因筛选标准,与非外伤性癫痫组比较,外伤性癫痫组样本中存在显著性表达差异的基因有192个,其中表达上调的有115个,表达下调的有77个。结论采用基因表达谱芯片技术可以有效地识别外伤性癫痫和非外伤性癫痫的差异表达基因,并可用于外伤性癫痫发病机制和分子分型的研究。
Objective To compare the gene expression profiles of traumatic and non-traumatic epileptic foci to explore the pathogenesis of traumatic epilepsy and provide evidence for the related identification. Methods Totally 15 cases of traumatic and non-traumatic epileptic foci were collected. Total RNA was separately extracted and hybridized with human cDNA microarray by reverse transcriptase-labeled probes to obtain the scanned images. The intensity and ratio of the signals were analyzed, and the experimental data were normalized by RNA microarray analysis, and the differentially expressed genes were clustered analysis. Results A total of more than 2 990 genes were expressed in the two samples (expressed more than 2 ~ 5 times more than the background). In 30% of the samples, the level of expression was greater than 1.5 times as the differential gene screening criteria. Compared with the non-traumatic epilepsy group, there were 192 genes with significant difference in traumatic epilepsy group samples, of which 115 , 77 were down-regulated. Conclusion The gene expression microarray technique can effectively identify differentially expressed genes in traumatic epilepsy and non-traumatic epilepsy, and can be used to study the pathogenesis and molecular typing of traumatic epilepsy.