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目的 探讨p5 3、p16蛋白及增殖细胞核抗原 (PCNA)异常表达与胃癌生物学行为的关系。方法 应用免疫组化ABC法 ,对 95例胃癌进行p5 3、p16蛋白表达产物和PCNA进行检测。结果 胃癌组织中p5 3、p16、PCNA阳性率分别为 49 5 % ( 4 7/ 95 )、2 0 0 % ( 19/95 )、78 9% ( 75 / 95 )。p5 3蛋白、PCNA在进展期胃癌 ( 5 3 5 %、82 6% )淋巴结阳性胃癌 ( 5 9 3 %、86 4% )表达率均高于早期胃癌( 11 1%、44 4% )和淋巴结阴性胃癌 ( 3 3 3 %、66 7% ) (P <0 0 5 )。p5 3蛋白、PCNA在累及浆膜胃癌的表达率高于局限粘膜及粘膜下层胃癌 (P <0 0 5 ,P <0 0 1)。PCNA异常表达与胃癌组织学分型有关 (P <0 0 5 )。p16蛋白表达与胃癌大多数生物学行为无明显关系 ,但其在淋巴结阳性胃癌中的表达率 ( 11 9% )低于淋巴结阴性胃癌中的表达率 ( 3 3 3 % ) (P <0 0 5 )。p5 3阳性组织大多数伴PCNA阳性表达 (P <0 0 1)。结论 p5 3蛋白、PCNA异常表达在胃癌浸润转移过程及增殖中均起重要作用。p16蛋白表达缺失可能是胃癌淋巴结转移的重要促发因素。
Objective To investigate the relationship between the abnormal expression of p53, p16 protein and proliferating cell nuclear antigen (PCNA) and the biological behavior of gastric cancer. Methods Immunohistochemical ABC method was used to detect the expressions of p53, p16 protein and PCNA in 95 cases of gastric cancer. Results The positive rates of p5 3, p16 and PCNA in gastric cancer tissues were 49 5% (47/95), 200% (19/95) and 78 9% (75/95), respectively. The expression rates of p53 protein and PCNA in advanced gastric cancer (53.3%, 86.4%) were significantly higher than those in advanced gastric cancer (11.1%, 44.4%) and lymph node Negative gastric cancer (33.3%, 66.7%) (P <0 05). The expression of p53 protein and PCNA in gastric and gastric mucosa was higher than that in localized mucosa and submucosal gastric cancer (P <0.05, P <0.01). The abnormal expression of PCNA correlated with the histological type of gastric cancer (P <0.05). The expression of p16 protein was not related to most biological behaviors of gastric cancer, but the expression of p16 protein was lower in lymph node positive gastric cancer (11 9%) than in lymph node negative gastric cancer (333%) (P <0.05 ). The majority of p53 positive tissues were accompanied by PCNA positive expression (P <0.01). Conclusion The abnormal expression of p53 protein and PCNA plays an important role in the invasion and metastasis of gastric cancer and in the proliferation of gastric cancer. The loss of p16 protein expression may be an important factor for gastric cancer lymph node metastasis.