Objective To clone the full-length cDNA of a gene responsible for vascular smooth muscle cell (v-SMC) proliferation in atherogenesis, and study its function.Methods Oxidized low density lipoprotein (ox-LDL) at optimal concentration was used as the stimulant to induce v-SMC prol iferation in culture medium. AcDNA subtractive library of v-SMC proliferation specific toox-LDL stimulation was established using subtractive hybridization technique. Methods, including blotting, North hybridization and gene sequen cing, were used to clone new gene fragments. By using full-length cDNA screeni ng and protein expression techniques, one full-length cDNA was cloned and its f unction was studied. Results One full-length cDNA was cloned. The new gene (Genbank AF 174647) expressed a 44 kDa protein, which might be associated with the activity of ox-LDL. Conclusion The new gene cloned may be associated with SMC proliferation in atherogenesis.