研究目的:通过实验研究探索单克隆抗体用于流行性乙型脑炎特异性治疗的安全性与有效性。研究方法:采用淋巴细胞杂交瘤技术制备、筛选出针对该病毒E蛋白中和抗原表位的单抗;建立恒河猴乙脑感染发病模型;一次性应用纯化的混合单抗对感染小鼠、幼羊和恒河猴进行实验性治疗;选择诊断确切的中、重型乙脑志愿者作临床疗效观察。研究结果:表明单抗的中和作用在治疗中起主 Objectives: To explore the safety and efficacy of monoclonal antibodies for the specific treatment of Japanese encephalitis by experimental studies. Methods: The lymphocyte hybridoma technique was used to prepare and screen out the monoclonal antibodies against neutralizing epitopes of E protein of the virus. The etiological model of macaque JE was established. The purified monoclonal antibody was used to treat the infected mice, Young sheep and rhesus monkeys for experimental treatment; select the diagnosis of moderate and severe hepatitis B volunteers for clinical efficacy. The results show that the neutralizing effect of MAb plays a major role in the treatment