论文部分内容阅读
目的：探讨ｂｃｌ２ 和ｐ５３ 蛋白的表达与端粒酶活性的相关性及其与 Ｈ Ｃ Ｃ 发生的关系。方法：利用端粒酶原位标记法显示端粒酶活性，采用 Ｓ Ｐ 法免疫组化技术检测ｂｃｌ２ 和ｐ５３ 蛋白。结果：端粒酶在 Ｈ Ｃ Ｃ 中的阳性率（９１７ ％ ） 显著高于癌旁肝组织（５８３ ％ ）（ Ｐ＜ ００５） ，端粒酶活性强度与 Ｈ Ｃ Ｃ 分化程度无关（ Ｐ＞ ００５） ；癌组织中ｂｃｌ２ 和ｐ５３ 蛋白的阳性率均高于癌旁组织（ Ｐ＜ ００１） ； Ｈ Ｃ Ｃ 和癌旁组织中端粒酶活性程度随ｂｃｌ２ 蛋白表达增强而升高，并呈明显正相关，但与ｐ５３ 蛋白表达强度无明显相关性。结论：ｂｃｌ２ 蛋白的过度表达可能是端粒酶激活的重要途径之一，ｂｃｌ２ 蛋白过度表达可能通过激活端粒酶使肝细胞恶性转化导致 Ｈ Ｃ Ｃ 发生，而ｐ５３ 基因突变可能对端粒酶的激活无直接影响。
Objective: To investigate the relationship between the expression of bcl2 and p53 protein and telomerase activity and its relationship with the occurrence of H C C . Methods: Telomerase activity was detected by in situ labeling with telomerase, and bcl2 and p53 protein were detected by SP immunohistochemistry. Results: The positive rate of telomerase in H C C (91.7%) was significantly higher than that in para-cancerous liver tissue (58. 3%) (P < 0. 05). Telomerase activity intensity and H C C differentiation. The degree was not related (P> 005); the positive rates of bcl2 and p53 protein in cancer tissues were higher than those in adjacent tissues (P<001); the degree of telomerase activity in HCC and paracancerous tissues followed The expression of bcl2 protein was increased and increased, and there was a significant positive correlation. However, there was no significant correlation with the expression of p53 protein. Conclusion: Overexpression of bcl2 protein may be one of the important pathways for telomerase activation. Overexpression of bcl2 protein may lead to the malignant transformation of hepatocytes through the activation of telomerase, leading to the occurrence of H C C. The p53 gene mutation may be Telomerase activation has no direct effect.