Acacia karroo(A.karroo) has been used as herbal medicine by the indigenous people of southern Africa for several centuries.The potential of.4.karroo as herbal medicine,its associated phytochemistry and biological activities are reviewed.The extensive literature survey revealed that A.karroo is traditionally used to treat or manage 32 and five human and animal diseases and ailmenls.respectively.The species is used as herbal medicine for diseases and ailments such as colds,diarrhoea,dysentery,flu.malaria,sexually transmitted infections(STis),wounds,and also as colic and ethnovetcrinary medicine.Multiple classes of phytochemicals such as flavonoids.phenols,phytosterols,proanthocyanidin.tannin,terpenes as well as several minerals have been identified from leaves and roots of A.karroo.Scientific studies on A.karroo indicate that it has a wide range of pharmacological activities which include antibacterial,antifungal,antigonococcal.antihelmintic,antilisterial,antimalarial,antimycobacterial,antioxidant.HIV-1 reverse transcriptase,anti-inflammatory and analgesic.Acacia karroo has a lot of potential as a possible source of pharmaceutical products for the treatment of a wide range of both human and animal diseases and ailments.Future research should focus on the mechanisms of action of the different plant parts used as herbal medicines.isolated compounds,their efficacy,toxicity and clinical relevance. Acacia karroo (A.karroo) has been used as herbal medicine by the indigenous people of southern Africa for several centuries.The potential of.4.karroo as herbal medicine, its associated phytochemistry and biological activities are reviewed. Extensive literature survey revealed that A. karroo is traditionally used to treat or manage 32 and five human and animal diseases and ailmenls. Specifically. The species is used as herbal medicine for diseases and ailments such as colds, diarrhea, dysentery, flu. Malaria, sexually transmitted infections (STis ), wounds, and also as colic and ethnovetcrinary medicine. Multiple classes of phytochemicals such as flavonoids. phenols, phytosterols, proanthocyanidin.tannin, terpenes as well as several minerals have been identified from leaves and roots of A. karroo. Scientific studies on A .karroo indicate that it has a wide range of pharmacological activities which include antibacterial, antifungal, antigonococcal. antihelmintic, antilisterial, antimalarial, antimycobacterial, a ntioxidant. HIV-1 reverse transcriptase, anti-inflammatory and analgesic. Acacia karroo has a lot of potential as a possible source of pharmaceutical products for the treatment of a wide range of both human and animal diseases and ailments. Future research should focus on the mechanisms of action of the different plant parts used as herbal medicines .isolated compounds, their efficacy, toxicity and clinical relevance.