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目的研究甲基对硫磷检测的快速检测方法。方法 用多孔碳球材料修饰硼掺杂金刚石(BDD)电极,将乙酰胆碱酯酶(ACh E)固定在修饰电极表面制得酶传感器,用于甲基对硫磷的检测。对所制备的酶传感器进行电化学表征,对实验参数进行优化。结果 底物氯化乙酰胆碱的产物在ACh E/BDD电极和ACh E/多孔碳球/BDD电极上的氧化峰电流分别为0.4930μA和1.102μA,峰电流提高了123.53%。在ACh E/多孔碳球/BDD电极上,建立抑制率与甲基对硫磷浓度的负对数的对应关系,在浓度范围10-10~10-7g/L内呈良好的线性关系,线性方程为Y=-13.20297X+148.375,相关系数R2为0.9985。按抑制率为10%计算检出限为3.02×10-12g/L。对实际样品黄瓜汁进行检测,样品回收率为92.60%~102.00%。结论 此方法简单、快速、灵敏度高,适合用于实际样品中的甲基对硫磷残留分析。
Objective To study the rapid detection of methyl parathion. Methods Boron doped diamond (BDD) electrodes were modified with porous carbon spheres and acetylcholinesterase (AChE) was immobilized on the surface of the modified electrode to prepare an enzyme sensor for the determination of methyl parathion. The prepared enzyme sensor was electrochemically characterized and the experimental parameters were optimized. The results showed that the oxidation peak currents of ACh E / BDD electrode and ACh E / porous carbon ball / BDD electrode were respectively 0.4930μA and 1.102μA, and the peak current increased by 123.53%. On the ACh E / porous carbon ball / BDD electrode, the corresponding relationship between the inhibition rate and the negative logarithm of methyl parathion concentration was established, showing a good linear relationship in the concentration range of 10-10 ~ 10-7g / L. The linearity The equation is Y = -13.20297X + 148.375, the correlation coefficient R2 is 0.9985. The detection limit was 3.02 × 10-12g / L when the inhibition rate was 10%. The actual sample cucumber juice testing, sample recovery rate of 92.60% ~ 102.00%. Conclusion This method is simple, rapid and sensitive and suitable for the analysis of parathion residues in real samples.