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目的探讨黏蛋白3A(MUC3A)在肝外胆管癌(ECC)组织中的表达及其临床意义。方法收集手术切除的ECC癌组织标本35份(35例),以手术切缘阴性组织为正常组织标本20份(20例)。采用链霉素亲和生物素-过氧化物酶免疫组化方法(SP法)检测并对比MUC3A在胆管癌组织和正常胆管组织中的表达情况,同时收集35例ECC患者的临床病理资料,分析MUC3A与临床病理特性之间的相关性。结果 MUC3A在肿瘤组织癌巢中高表达,在肿瘤细胞的胞浆中显示出高信号,同时癌巢中的管腔内也可见到不同程度的免疫染色。MUC3A在ECC癌组织中的阳性表达率为85.7%,在正常胆管组织中的阳性表达率为35.0%,两者差异有统计学意义(<0.01)。胆管癌组织中MUC3A的表达与性别、年龄、肿瘤部位方面均无关(均>0.05);MUC3A在癌组织低分化或未分化、有淋巴结转移、有周围组织浸润、UICC分期Ⅲ~Ⅳ期中的阳性表达率均高于癌组织的中分化或高分化、无淋巴结转移、无周围组织浸润、UICC分期Ⅰ~Ⅱ期(均<0.05)。结论 MUC3A在胆管癌组织中高表达,并与病理类型、淋巴结转移、肿瘤分期密切相关,MUC3A蛋白高表达提示ECC患者预后不良。
Objective To investigate the expression and clinical significance of MUC3A in extrahepatic cholangiocarcinoma (ECC) tissues. Methods 35 cases (35 cases) of surgical resected ECC tissues were collected, 20 cases (20 cases) were normal tissue specimens with negative margins of surgical margins. The expression of MUC3A in cholangiocarcinoma and normal biliary tissues was detected by streptavidin-biotin-peroxidase immunohistochemistry (SP method). Meanwhile, the clinical pathological data of 35 patients with ECC were collected and analyzed Correlation between MUC3A and clinicopathological features. Results MUC3A was highly expressed in tumor nests and showed high signal in the cytoplasm of tumor cells. Different degrees of immunostaining were found in the lumen of cancer nests. The positive expression rate of MUC3A in ECC tissues was 85.7%, while it was 35.0% in normal biliary tissues. The difference was statistically significant (<0.01). The expression of MUC3A in cholangiocarcinoma was not related to gender, age and tumor location (all> 0.05). MUC3A was highly differentiated or undifferentiated in cancer tissues, with lymph node metastasis, infiltrating surrounding tissues and positive in stage Ⅲ ~ Ⅳ of UICC The expression rates were higher than those in moderately differentiated or well-differentiated cancerous tissues, with no lymph node metastasis and no infiltration of surrounding tissues. The UICC stages Ⅰ ~ Ⅱ (all <0.05). Conclusions MUC3A is overexpressed in cholangiocarcinoma and closely related to the pathological type, lymph node metastasis and tumor stage. The high expression of MUC3A protein suggests that the prognosis of ECC patients is poor.