抗凝血酶Ⅲ(AT-Ⅲ)与肝素之间的相互作用具有高度特异性,并对凝血酶以及活化的因子Ⅸ及Ⅹ等的抑制速度,产生增强的效应。AT与肝素的这种相互作用,使肝素亲和层析法成为分离制备AT的重要方法。但通常商品肝素中具有高亲和力的部分不到1/3,且其中能特异地结合AT的位点仅占2～3％。因而结合在琼脂糖上的肝素总量中,仅极小部分能结合AT,而绝大部分肝素则 The interaction between antithrombin III (AT-III) and heparin is highly specific and exerts an enhanced effect on the rate of inhibition of thrombin and activated factors IX and X and the like. This interaction between AT and heparin makes heparin affinity chromatography an important method for the separate preparation of AT. However, in general, there are less than 1/3 of the high affinity heparin in the product, and only 2 to 3% of the sites can specifically bind AT. Thus combined with the total amount of heparin on agarose, only a very small part of AT can bind, and most of the heparin