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目的了解CXCR2和CXCR4对HeLa细胞迁移、增殖和分泌VEGF的影响。方法 Transwell检测HeLa细胞迁移情况;MTT法检测CXCR2和CXCR4对HeLa细胞增殖的影响;ELISA法检测HeLa细胞VEGF表达情况。结果敲除CXCR2或CXCR4后,宫颈癌HeLa细胞迁移率分别为(2.1±0.8)和(2.6±0.9),未敲除组迁移率为(3.6±1.1),差异有统计学意义(P<0.05);激活CXCR2或CXCR4后,HeLa细胞增殖率分别为(86.1±15.3)%和(83.9±15.9)%,未激活组HeLa细胞增殖率为(66.7±8.7)%(P<0.05),激活CXCR2或CXCR4后,VEGF表达水平分别为(6.84±0.19)和(6.59±0.14)pg/L,未激活组为(0.74±0.03)pg/L(P<0.05)。结论HeLa细胞表面高表达的CXCR2和CXCR4在诱导HeLa细胞迁移、增殖和分泌VEGF方面具有重要的作用。
Objective To investigate the effects of CXCR2 and CXCR4 on the migration, proliferation and VEGF secretion of HeLa cells. Methods Transwell assay was used to detect the migration of HeLa cells. The effect of CXCR2 and CXCR4 on the proliferation of HeLa cells was detected by MTT assay. The expression of VEGF in HeLa cells was detected by ELISA. Results After CXCR2 or CXCR4 knockdown, the rates of cervical HeLa cell migration were (2.1 ± 0.8) and (2.6 ± 0.9), respectively, and those in the untreated group were (3.6 ± 1.1), with significant difference (P <0.05 ). The proliferation rates of HeLa cells were (86.1 ± 15.3)% and (83.9 ± 15.9)%, respectively. The proliferation rate of HeLa cells was (66.7 ± 8.7)% (P <0.05) after activation of CXCR2 or CXCR4. Or CXCR4, the expression levels of VEGF were (6.84 ± 0.19) and (6.59 ± 0.14) pg / L, respectively, and those in inactive group were (0.74 ± 0.03) pg / L and Conclusion The high expression of CXCR2 and CXCR4 on the surface of HeLa cells play an important role in inducing HeLa cells to migrate, proliferate and secrete VEGF.