作者在扫描电镜下,对体外培养的人胎儿心肌细胞形态进行了观察。材料取自10、13、16周引产胎儿心脏。首先进行心室肌组织块培养(培养基:0.98％199、15～20％(小牛血清、青、链霉素100IU.100μg/ml;pH7.0±、37℃恒温箱培养),然后将培养块上新生并脱落贴壁生长的心肌细胞原位制成扫描电镜标本进行观察。心肌细胞生长早期(脱落生长1周左右),细胞呈球形。每个细胞表面的突起形 Under the scanning electron microscope, we observed the morphology of human fetal cardiomyocytes cultured in vitro. Materials were taken from fetuses at 10, 13 and 16 weeks of pregnancy. Ventricular muscle tissue culture was first carried out (culture medium: 0.98% 199,15-20% (calf serum, cyan, streptomycin 100IU.100μg / ml; pH7.0 ± 37 ° C incubator) The cardiomyocytes, which were newly born and detached, were observed in situ by scanning electron microscopy (SEM) .In the early stage of cardiomyocyte growth (about 1 week after exfoliation), the cells were spherical.The protuberances on the surface of each cell