论文部分内容阅读
目的探讨多重PCR技术用于检测牙龈卟啉单胞菌(Porphyromonasgingivalis,P.gingivalis)致病岛rag基因的可行性,并研究致病岛rag基因在毒力株和非毒力株中的分布情况。方法本研究于2011年1—6月在山东省口腔生物医学重点实验室进行,对P.gingivalis毒力株和非毒力株进行厌氧培养,采用普通PCR和多重PCR分别对他们的致病岛rag基因进行检测,对比检测结果,并对临床标本进行预实验研究。结果普通PCR和多重PCR均能对P.gingivalis致病岛rag基因进行检测,并且结果一致。多重PCR可用于临床标本的检测。致病岛rag基因不同基因型在毒力株和非毒力株中的分布不同,rag-1型存在于高毒力株P.gingivalisW83中,而rag-4型存在于低毒力株P.gingivalisATCC33277中。结论致病岛rag基因与细菌致病性密切相关;多重PCR技术省时省力、简单快速,为后续临床标本中rag基因的快速检测提供实验依据。
Objective To investigate the feasibility of multiplex PCR technique for the detection of rag gene in pathogenicity island of Porphyromonas gingivalis (P. gingivalis) and to study the distribution of rag gene in virulent strain and virulent strain . Methods The study was conducted in Key Laboratory of Oral Biomedicine of Shandong Province from January to June in 2011. Anaerobic culture of virulent strains of P. gingivalis and non-virulent strains was carried out anaerobically. The common and multiplex PCR were used to detect their pathogenicity Island rag gene detection, comparison test results, and pre-experimental study of clinical specimens. Results Both PCR and multiplex PCR could detect rag gene of P. gingivalis, and the results were the same. Multiplex PCR can be used for the detection of clinical specimens. The different genotypes of the rag gene of pathogenicity island are distributed in virulent and non-virulent strains, rag-1 is in P. gingivalis W83, while rag-4 is in virulent P. gingivalis ATCC33277. Conclusion The rag gene of pathogenicity island is closely related to the pathogenicity of bacteria. The multiplex PCR technique is time-saving and labor-saving, which is simple and rapid. It provides an experimental basis for the rapid detection of rag gene in subsequent clinical specimens.