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目的探讨木鳖子单体化合物对羟基桂皮醛(p-hydroxycinnamaldehyde,PHD)对黑素瘤B16细胞体内生长的抑制作用。方法应用MTS法检测PHD对B16细胞增殖的影响,倒置相差显微镜下观察形态变化。构建黑素瘤B16细胞小鼠皮下移植瘤模型,分为2组:治疗组小鼠ip 2 mg/kg PHD,模型组注射相同体积的生理盐水。观察2组小鼠成瘤情况,并测量肿瘤体积和质量;免疫组织化学法检测肿瘤组织中酪氨酸酶(Tyr)、S-100B、MMP-9、p-P38和p-ERK蛋白的表达变化;苏木精-伊红染色法(HE)分析小鼠肝和肺组织的形态学变化。结果 PHD对黑素瘤B16细胞具有明显的生长抑制作用(P<0.01)。20μmol/L PHD处理B16细胞48 h后,细胞数量减少,呈典型分化形态。PHD治疗组的肿瘤体积和质量均明显低于模型组(P<0.01)。与模型组相比,治疗组小鼠肿瘤组织中Tyr和p-P38蛋白的表达水平升高,MMP-9、S-100B和p-ERK蛋白表达水平则明显降低。治疗组小鼠肝和肺无明显组织形态学改变,模型组小鼠肺部有肿瘤转移。结论 PHD对小鼠体内黑素移植瘤的生长具有显著抑制作用。
OBJECTIVE To investigate the inhibitory effect of p-hydroxycinnamaldehyde (PHD) on the growth of melanoma B16 cells in vitro. Methods The effect of PHD on the proliferation of B16 cells was detected by MTS method. Morphological changes were observed under inverted phase contrast microscope. The subcutaneously transplanted tumor model of melanoma B16 cells was established and divided into two groups: the mice in the treatment group were treated with ip 2 mg / kg PHD, and the model group was injected with the same volume of saline. The tumorigenicity of the two groups of mice was observed and the tumor volume and quality were measured. The expression of tyrosinase (Tyr), S-100B, MMP-9, p-P38 and p-ERK in tumor tissues were detected by immunohistochemistry The changes of liver and lung tissue were analyzed by hematoxylin-eosin staining (HE). Results PHD had obvious growth inhibitory effect on melanoma B16 cells (P <0.01). After treated with 20 μmol / L PHD for 48 h, the number of cells decreased, showing a typical differentiation pattern. The tumor volume and quality of PHD treatment group were significantly lower than the model group (P <0.01). Compared with the model group, the expression levels of Tyr and p-P38 protein in the tumor tissue of the treated mice increased, while the expression of MMP-9, S-100B and p-ERK protein decreased significantly. The mice in the treatment group showed no obvious morphological changes in the liver and lungs, and the mice in the model group had tumor metastasis in the lungs. Conclusion PHD has a significant inhibitory effect on the growth of melanoma in mice.