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目的研究TGF-β1基因+915位点G/C多态性与胃癌的相关性。方法采用聚合酶链反应-限制性片段长度多态性方法 ,检测80例胃癌患者与102例正常对照者TGF-β1基因+915位点G/C等位基因及基因型分布,分析该基因多态性与胃癌幽门螺杆菌感染的相关性。采用酶联免疫吸附试验(ELISA)检测20例胃癌患者和20例正常对照者血清TGF-β1水平。结果 TGF-β1等位基因频率及基因型频率在病例组和对照组的分布差异无统计学意义;胃癌按幽门螺杆菌感染分层后,TGF-β1基因型及等位基因频率分布差异亦无统计学意义,胃癌患者血清TGF-β1水平显著高于对照组。结论 TGF-β1+915位点基因多态性与胃癌无关。
Objective To study the relationship between G-C polymorphism of TGF-β1 +915 and gastric cancer. Methods The alleles and genotypes of G / C at +915 locus of TGF-β1 gene in 80 gastric cancer patients and 102 normal controls were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) Correlation between Helicobacter pylori infection and gastric cancer. Serum levels of TGF-β1 were detected by enzyme-linked immunosorbent assay (ELISA) in 20 gastric cancer patients and 20 normal controls. Results There were no significant differences in the distribution of TGF-β1 allele frequency and genotype between the case group and the control group. There was no difference in the distribution of TGF-β1 genotype or allele frequency in gastric cancer after H. pylori infection Statistical significance, serum TGF-β1 levels in patients with gastric cancer was significantly higher than the control group. Conclusion The gene polymorphism of TGF-β1 + 915 locus has nothing to do with gastric cancer.