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目的:探讨子宫颈癌组织中P19ARF和P18INK4c基因的表达及临床意义。方法:采用免疫组织化学方法 (SP法)、逆转录-聚合酶链式反应(RT-PCR)方法检测47例子宫颈癌组织、20例CIN、20例正常子宫颈组织中P19ARF、P18INK4c的表达水平并进行比较分析。结果:①P19ARFmRNA在宫颈癌中的表达水平明显低于CIN组和正常组(P<0.05),P18INK4cmRNA在宫颈癌中的表达水平明显高于CIN组和正常组(P<0.05);②P19ARF蛋白在正常宫颈、CIN、宫颈癌中的阳性表达率逐渐降低(P<0.05),P18INK4c蛋白在正常宫颈、CIN、宫颈癌中的阳性表达率逐渐升高(P<0.05);③P19ARF蛋白在宫颈癌中的阳性表达率仅与淋巴结转移有关(P<0.05),其在宫颈癌I期中的阳性表达率高于II期和III+IV期(P>0.05);P18INK4c蛋白在宫颈癌中的阳性表达率与临床分期、淋巴结转移有关,与病理学分级、组织学类型无关(P>0.05);④P19ARF和P18INK4c的表达之间无显著相关性(P>0.05)。结论:P19ARF、P18INK4c的异常表达很可能参与了宫颈癌的发生、发展过程。两种基因表达没有显著相关性。
Objective: To investigate the expression of P19ARF and P18INK4c in cervical cancer and its clinical significance. Methods: The expressions of P19ARF and P18INK4c in 47 cases of cervical cancer, 20 cases of CIN and 20 cases of normal cervical tissue were detected by immunohistochemistry (SP method) and reverse transcription polymerase chain reaction (RT-PCR) And comparative analysis. Results: ① The expression of P19ARF mRNA in cervical cancer was significantly lower than that in CIN and normal controls (P <0.05). The expression of P18INK4 mRNA in cervical cancer was significantly higher than that in CIN and normal controls (P <0.05) The positive rates of P18INK4c protein in cervical, CIN and cervical cancer were gradually decreased (P <0.05). The positive rates of P19ARF protein in cervical cancer The positive rate of P18INK4c in cervical cancer was significantly higher than that of stage II and III + IV (P> 0.05) (P> 0.05). There was no significant correlation between the expression of P19ARF and P18INK4c (P> 0.05) .Conclusion: There is no significant correlation between the expression of P19ARF and P18INK4c in clinical stage, lymph node metastasis and histological type. Conclusion: The abnormal expression of P19ARF and P18INK4c may be involved in the occurrence and development of cervical cancer. There was no significant correlation between the two gene expressions.