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目的探讨神经内分泌分化对前列腺癌细胞生长的作用及对雄激素受体表达的影响。方法建立神经内分泌分化的前列腺癌细胞模型PC3MNE和LNCaPNE;采用甲基噻唑基四唑(MTT)试验观察其调节前列腺癌细胞生长的作用[以吸光度值(A)表示];采用逆转录聚合酶链反应和Western杂交方法检测LNCaPNE对LNCaP细胞雄激素受体表达的影响。结果PC3MNE的培养上清液可促进PC3M细胞的生长(A值在培养24h为034±018与050±009,48h为038±016与057±009,72h为038±015与055±005,P均<005);在有雄激素时,LNCaPNE的培养上清液不促进LNCaP细胞的生长,对其雄激素受体的表达也没有显著影响;去除雄激素后,LNCaPNE的培养上清液可促进LNCaP细胞的生长,并能下调其雄激素受体的表达(P均<005)。结论在雄激素阻断后,神经内分泌分化的前列腺癌细胞能以旁分泌的方式支持其他前列腺癌细胞生长,降低其雄激素受体的表达。
Objective To investigate the effect of neuroendocrine differentiation on the growth of prostate cancer cells and its effect on the expression of androgen receptor. Methods PC3MNE and LNCaPNE were used to establish the model of neuroendocrine differentiation of prostate cancer cells. MTT assay was used to observe the effect of PC3MNE on the growth of prostate cancer cells [A (absorbance value)]. The reverse transcriptase polymerase chain reaction The effect of LNCaPNE on androgen receptor expression in LNCaP cells was detected by Western blot and Western blot. Results The culture supernatant of PC3MNE could promote the growth of PC3M cells. The values of A were 034 ± 018 and 050 ± 009 at 24h, 038 ± 016 and 057 ± 009 at 48h, 038 ± 015 and 055 ± 005 at 72h and P <005). In the presence of androgen, LNCaPNE culture supernatant did not promote the growth of LNCaP cells, and its androgen receptor expression had no significant effect; removal of androgen, LNCaPNE culture supernatant can promote LNCaP Cell growth and down-regulated the expression of androgen receptor (all P <005). Conclusion After androgen blockade, the neuroendocrine differentiated prostate cancer cells can support the growth of other prostate cancer cells in a paracrine manner and decrease their androgen receptor expression.