论文部分内容阅读
目的 探讨外周血甲胎球蛋白 (alpha fetoprotein ,AFP)基因分析对肝癌诊断的临床价值。方法 从人肝癌组织和外周血单核细胞中制备总RNA ,经随机引物和逆转录酶合成cDNA后 ,以巢式聚合酶链反应 (polymerasechainreaction ,PCR)扩增AFP基因片段 ,分析其在肝癌诊断中的临床应用价值。结果 所设计反转录巢式PCR扩增AFP基因片段为 15 9bp ,方法的灵敏度为 5 μg/L ;AFP基因在肝癌的癌灶组织、癌灶周围组织和远离癌灶肝组织中的检出率分别为 10 0 % (2 0 /2 0 )、6 5 %(13/2 0 )和 0 % (0 /2 0 ) ;在肝癌患者外周血中的阳性率为 5 4% (36 /6 7) ,明显高于其他肝病组和肝外肿瘤组 ;在Ⅰ、Ⅱ和Ⅲ期肝癌中阳性率分别为 33% (3/9)、2 6 % (5 /19)和 72 % (2 8/39) ;伴肝外转移肝癌 4例和术后复发肝癌 12例全部阳性 ;AFP基因与肝癌体积间无明显相关性。结论 分析外周血AFP mRNA有助于肝癌诊断、肝癌转移或术后复发的监测。
Objective To investigate the clinical value of peripheral blood alpha fetoprotein (AFP) gene analysis in the diagnosis of hepatocellular carcinoma. Methods Total RNA was prepared from human hepatocellular carcinoma and peripheral blood mononuclear cells. cDNA was synthesized by random primers and reverse transcriptase. AFP gene fragment was amplified by nested polymerase chain reaction (PCR) to analyze the diagnosis of hepatocellular carcinoma. The clinical application value. Results The AFP gene fragment amplified by reverse transcription nested PCR was designed to be 149 bp, and the sensitivity of the method was 5 μg/L. The AFP gene was detected in the foci of liver cancer, the tissues around the foci, and the tissues away from the foci. The rates were 100% (20/20), 65% (13/20%), and 0% (0/20); the positive rate in peripheral blood of HCC patients was 54% (36/6). 7) was significantly higher than other liver disease and extrahepatic tumors; the positive rates in stage I, II, and III liver cancers were 33% (3/9), 26% (5/19), and 72% (2 of 8). /39) ; There were 4 cases with extrahepatic metastatic liver cancer and 12 cases with recurrence of liver cancer after operation. There was no significant correlation between the AFP gene and the volume of liver cancer. Conclusion Analysis of AFP mRNA in peripheral blood is helpful for the diagnosis of liver cancer, liver cancer metastasis or postoperative recurrence monitoring.