目的　观察胆肠吻合愈合过程的超微结构变化 ,阐明良性胆管狭窄形成机制。方法　通过制作犬胆总管十二指肠吻合模型 ,分别于术后 1周、3周、3月、6月取材行透射电镜 (TEM )、扫描电镜 (SEM)观察 ,并行计算机图像分析测定细胞外胶原容积密度 (ECVD)变化。结果　胆道粘膜上皮修复较差 ,肌成纤维细胞功能活跃 ,持续存在于整个愈合过程 ,细胞外基质过度沉积。结果导致瘢痕性挛缩 ,吻合口狭窄。结论　肌成纤维细胞是导致胆肠吻合口瘢痕性挛缩的主要原因 Objective To observe the ultrastructural changes during the process of gallbladder anastomosis and elucidate the mechanism of benign biliary stricture. Methods The model of duodenal anastomosis of canine bile duct was made. The specimens were examined by transmission electron microscope (TEM) and scanning electron microscope (SEM) at 1 week, 3 weeks, 3 months and 6 months after operation respectively. Collagen bulk density (ECVD) changes. Results Poor biliary mucosal epithelial repair, active myofibroblasts, persist in the entire healing process, excessive deposition of extracellular matrix. The result of cicatricial contracture, anastomotic stenosis. Conclusion Myofibroblasts are the main cause of cicatricial contracture in cholangio-enterostoma