论文部分内容阅读
A novel micelle to solvent stacking on-line sample preconcentration technique in capillary zone electrophoresis(MSS-CZE) has been developed to determine the strychnine and brucine in traditional Chinese medicine preparations.The optimal running buffer was 30 mmol/L H_3PO_4 containing 20%acetonitrile at pH 4.0.The sample matrix was 8 mmol/L H_3PO_4 containing 5 mmol/L sodium dodecyl sulfate(SDS) at pH 3.0.The established MSS-CZE method afforded more than 50-fold improvements in concentration sensitivity compared with typical CZE-UV analysis.The calibration curve was linear in the range from 0.2 to 15.0μg/ mL for both strychnine and brucine,with correlation coefficients of 0.9984 and 0.9976,respectively.The limits of detection(5/ N = 3:1) for strychnine and brucine were 0.02 and 0.05μg/mL,respectively.The MSS-CZE method has been successfully applied to the analysis of strychnine and brucine in Chinese medicinal preparations.
A novel micelle to solvent stacking on-line sample preconcentration technique in capillary zone electrophoresis (MSS-CZE) has been determined to the strychnine and brucine in traditional Chinese medicine preparations. The optimal running buffer was 30 mmol / L H 3 PO 4 containing 20% acetonitrile at pH 4.0. The established matrix was 8 mmol / L H_3PO_4 containing 5 mmol / L sodium dodecyl sulfate (SDS) at pH 3.0. The established MSS-CZE method afforded more than 50-fold improvements in concentration sensitivity compared with typical CZE-UV analysis. The calibration curve was linear in the range from 0.2 to 15.0 μg / mL for both strychnine and brucine, with correlation coefficients of 0.9984 and 0.9976, respectively. These limits of detection (5 / N = 3: 1) for strychnine and brucine were 0.02 and 0.05 μg / mL, respectively. The MSS-CZE method has been successfully applied to the analysis of strychnine and brucine in Chinese medicinal preparations.