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目的研究二月兰籽水煎剂对可卡因致小鼠急性肝损伤的保护作用。方法 50只雄性ICR小鼠随机分为对照组、模型组、二月兰籽水煎剂低、中、高剂量组,每组10只。二月兰籽水煎剂经口给予折合成生药量分别为21.5、43.0和86.0 g/kg,对照组和模型组经口给予等量的蒸馏水,1次/d,连续4 d;在实验的第4天,模型组、二月兰籽水煎剂剂量组腹腔注射盐酸可卡因溶液(75 mg/kg),对照组腹腔注射等量的生理盐水。在染毒后24 h,所有动物眼眶内眦静脉取血,测定血清中谷丙转氨酶(GPT)、谷草转氨酶(GOT)和乳酸脱氢酶(LDH)的活性。留取肝组织测定丙二醛(MDA)和过氧化氢酶(CAT)的含量。留取部分肝组织做常规病理切片检查。结果与正常组比较,模型组小鼠血清GPT、GOT和LDH活性均显著上升,肝组织MDA含量升高,CAT含量明显下降。与模型组比较,低、中、高剂量的二月兰籽水煎剂组小鼠血清GPT、GOT和LDH活性均显著降低,并呈剂量依赖性。肝组织MDA含量明显下降,CAT含量显著升高。病理切片显示二月兰籽水煎剂能够明显减轻可卡因对肝组织的炎症性破坏。结论二月兰籽水煎剂对可卡因引起的急性肝损害有一定的保护作用。
Objective To study the protective effect of LanZhi decoction in February on acute hepatic injury induced by cocaine in mice. Methods Fifty male ICR mice were randomly divided into control group, model group and low-dose, middle-dose and high-dose red rapeseed decoction in February. In February, LanZhi decoction was administered orally in an amount of 21.5, 43.0 and 86.0 g / kg, respectively. The control group and model group were orally administered an equal amount of distilled water once a day for 4 days. In the experimental On the fourth day, the model group and LanZhou decoction dose group were intraperitoneally injected with cocaine hydrochloride solution (75 mg / kg), and the control group was injected with the same amount of saline. At 24 h post-exposure, all animals received orbital venous blood to measure the activity of GPT, GOT and LDH. The liver tissues were collected to determine the content of malondialdehyde (MDA) and catalase (CAT). Take some liver tissue routine routine biopsy. Results Compared with the normal group, the activities of GPT, GOT and LDH in the model group increased significantly, the content of MDA in the liver tissue increased and the content of CAT decreased obviously. Compared with the model group, the activity of GPT, GOT and LDH in the low, medium and high doses of Lancome decoction of mice were significantly decreased in a dose-dependent manner. Liver MDA content decreased significantly, CAT content increased significantly. Pathological sections showed that Lancome decoction in February could significantly reduce the inflammatory destruction of hepatic tissue by cocaine. Conclusion February Lanzi decoction has certain protective effect on cocaine-induced acute liver injury.