论文部分内容阅读
目的:探讨梗阻性黄疸(obstructive jaundice,OJ)时NF-κB的变化及其对免疫应答的影响.方法:60只Wistar♂大鼠随机分成3组:假手术组(SHAM组)、梗阻性黄疸组(CBDL组)和梗阻性黄疸+NF-κB抑制剂脯氨酸二硫化氨基甲酸酯(PDTC)组(PDTC组).每组术后7、14d分批(n=10)检测光镜下肝脏病理组织学,血清总胆红素(TB),谷丙转氨酶(ALT),内毒素(LPS)水平,肝组织促炎因子IL-1β、IL-6,抑炎因子IL-10以及NF-κB蛋白表达.结果:CBDL组7、14d大鼠均出现肝组织病理损伤,CBDL组较SHAM组血清TB、ALT、LPS增高(7d:140.14±10.17vs7.309±1.04,134.479±10.20vs35.79±3.76,189.33±11.05vs2.816±0.58;14d:194.608±12.73vs36.142±3.51,217.797±12.37vs7.321±1.03,292.816±14.53vs2.664±0.53,均P<0.01),肝组织IL-1β,IL-6,IL-10和NF-κB表达增强(均P<0.01),且14d较7d变化更为显著.PDTC组大鼠血清TB、ALT和肝组织IL-1β、IL-6、NF-κB表达在7d时相点时比CBDL组显著下降(P<0.01),而到14d时相点时比较CBDL组无明显变化;LPS和IL-10表达与CBDL组各时相点相比无明显差异.结论:梗阻性黄疸大鼠早期(7d)通过PDTC抑制NF-κB活化表达,可下调促炎因子的表达,减轻肝损.后期(14d)作用不明显,其机制可能是通过LPS、IL-10等其他途径所致.
Objective: To investigate the changes of NF-κB and its effect on immune response in obstructive jaundice (OJ) .Methods: Sixty Wistar rats were randomly divided into three groups: sham operation group (SHAM group), obstructive jaundice (CBDL group) and obstructive jaundice + PDTC group (PDTC group) .At the 7th and 14th day after operation, the light microscopy (n = 10) The levels of serum total bilirubin (TB), alanine aminotransferase (ALT), endotoxin (LPS), hepatic proinflammatory cytokines IL-1β, IL-6, Results: The pathological changes of hepatic tissue were observed in CBDL group on the 7th and 14th day, while the levels of serum TB, ALT and LPS in CBDL group were higher than those in SHAM group (7d: 140.14 ± 10.17vs7.309 ± 1.04,134.479 ± 10.20vs35. 79 ± 3.76, 189.33 ± 11.05 vs 2.816 ± 0.58; 14d: 194.608 ± 12.73 vs 36.142 ± 3.51, 217.797 ± 12.37 vs 7.321 ± 1.03, 292.816 ± 14.53 vs 2.664 ± 0.53, all P <0.01) The levels of IL-1β, IL-6, IL-10 and NF-κB were significantly increased in both groups (P <0.01) 6, the expression of NF-κB in the 7d phase point than the CBDL group decreased significantly (P <0.01), and to 14d phase There was no significant difference in the expression of LPS and IL-10 compared with CBDL group at each time point.Conclusion: PDTC can inhibit the expression of NF-κB in early stage of obstructive jaundice (7d) Inflammatory factor expression, reduce liver damage. Late (14d) effect is not obvious, the mechanism may be through LPS, IL-10 and other ways.