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目的:探讨急性心肌梗死(AMI)后外周血循环单个核细胞(MNC)中1型多聚二磷酸腺苷核糖合成酶(PARP-1)的活性与肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)水平的关系。方法:选取AMI组患者46例,稳定型心绞痛组10例,正常对照组10例。用三氯乙酸沉淀法测定MNC中PARP-1的活性;酶联免疫吸附测定法测定血浆中IL-10和TNF-α的水平。结果:所有AMI组患者MNC中PARP-1的活性均显著升高,为正常对照组的4.08倍、稳定型心绞痛组的4.47倍。所有AMI患者血浆中TNF-α、IL-10的水平均显著升高。AMI组患者外周血循环MNC中PARP-1的活性与血浆TNF-α、IL-10的水平呈正相关。结论:患者AMI后外周血血浆中TNF-α、IL-10水平的增高与MNC中PARP-1的活化显著相关。PARP-1可能是免疫炎症系统激活过程中的重要调节因子。
Objective: To investigate the relationship between the activity of PARP-1 type 1 and the levels of tumor necrosis factor-α (TNF-α) and leukocyte in peripheral blood mononuclear cells (MNC) after acute myocardial infarction Interleukin-10 (IL-10) levels. Methods: Forty-six patients with AMI, 10 patients with stable angina and 10 patients with normal control were enrolled. The activity of PARP-1 in MNC was determined by trichloroacetic acid precipitation method. The levels of IL-10 and TNF-α in plasma were determined by enzyme linked immunosorbent assay (ELISA). Results: The activity of PARP-1 in MNC of all AMI patients was significantly increased, which was 4.08 times that of the normal control group and 4.47 times of that of the stable angina pectoris group. Plasma levels of TNF-α and IL-10 in all patients with AMI were significantly increased. The activity of PARP-1 in MNC of AMI patients was positively correlated with the level of plasma TNF-α and IL-10. Conclusion: The increase of plasma TNF-α and IL-10 level after AMI in patients with AMI is significantly correlated with the activation of PARP-1 in MNC. PARP-1 may be an important regulator of immune-inflammatory system activation.