Effects of neoadjuvant chemotherapy on the quantitative expression of P-gp, LRP, MRP, GST-π in NSCLC

来源 :中国肺癌杂志 | 被引量 : 0次 | 上传用户:yuanjian2009
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Background and objective Neoadjuvant chemotherapy (NACT) plays an important role in systemic chemotherapy for non-small cell lung cancer (NSCLC). P-glycoprotein (P-gp), lung resistance related protein (LRP), multidrug resistance-associated protein (MRP) and glutathione S-transferase (GST-π) may be associated to drug resisitance to chemotherapy in NSCLC. The aim of this study is to investigate the expressions of P-gp, LRP, MRP and GST-π in samples from NSCLC patients before and after treatment with NACT, and their quantitative changes, so that to evaluate the influence of NACT on drug resistance to chemotherapy of NSCLC. Methods Total 92 specimens from 72 cases of NSCLC, including 52 samples of surgery excision from non-NACT-treated patients and 20 paired samples before and after NACT from the same patient,were studied. The expression of P-gp, LRP, MRP and GST-π was detected with tissue chip technique and immunohistochemistry. The quantitative analysis was carried out by computer image analysis system. Results In the samples before NACT, the positive rate of P-gp, LRP, MRP, GST-π expression was 66.67% (48/72),72.22% (52/72), 81.94% (59/72), 83. 33% (60/72), respectively. The expressive intensity of P-gp, LRP and GST-π was significantly stronger in adenocarcinoma than that in squamous cell carcinoma (P<0.05, P<0.001, P<0.001, respectively); there was no significant difference in the expression of MRP between adenocarcinoma and squamous cell carcinoma (P>0.05). In samples after treatment with NACT, the expression of P-gp, GST-π demonstrated by average optical density (AOD) and integral optical density (IOD) were significantly higher (P<0.05, P<0.001 respectively) than that in biopsied samples taken before NACT; The change in expression of P-gp, GST-π was also showed difference by different histopathological types, differentiations, ages, sizes, clinical stages as well as lymph node metastasis or not (P<0. 05 or P<0. 001). There was no significant difference between samples taken before and after NACT (P>0.05) in the expression of LRP and MRP demonstrated by both of AOD and IOD. Conclusion The results suggest that drug resistance in adenocarcinoma is primarily stronger than that in squamous cell carcinoma. NACT may enhance acquired drug resistance of NSCLC through inducing the expression of drug resistance protein. The results indicate that acquired drug resistance must be considered with the application of NACT to NSCLC patient in clinic, especially to patient in stage Ⅰ and Ⅱ. Since NACT may lead to the enhancement of acquired drug resistance in stage Ⅰ and Ⅱ , this may dwindle the therapeutic effect of chemotherapy after surgery. Comparative examination of drug resistance proteins before and after NACT, combining with comprehensive consideration of chemical regimens of NACT, should be recommended during chemotherapy of NSCLC for both before and after surgery.
其他文献
Objective: To compare the curative effectiveness of continuous transarterial infusion chemotherapy and systemic venous chemotherapy in treating patients with ad
目的探讨老年人上消化道出血的临床特点。方法回顾性分析我院1997年1月~2006年12月间收治的老年人上消化道出血198例的临床特点,并与同期中青年病例比较。结果老年人上消化道
目的 探讨重型颅脑损伤性高血糖患者血浆肿瘤坏死因子-α(TNF-α)与胰岛β-细胞功能不全和胰岛素抵抗之间的关系.方法 观察我院近年来50例重型颅脑损伤性高血糖患者血浆TNF-
目的 分析早产儿视网膜病变(retinopathy of prematurity,ROP)的发病情况,为防治ROP提供参考.方法 对2005年1月1日~12月31日在北京海淀区妇幼保健院出生的孕周≤34周或出生体重≤2 000 g的早产儿生后4~6周开始定期检查眼底至周边视网膜血管化.根据早产儿视网膜病变国际分期标准进行诊断、分期.结果 75例资料完整的早产儿中有5例发生ROP,患病率为6.7%,
马兜铃酸肾病是一种药物性肾损害疾病.国内外学者对其研究已经十余年,但其发病机制目前尚不十分清楚,存在多种观点和假说.本文参阅了国内外相关文献,着重就该病的发病机制简
目的:探讨采用拔除磨牙的方法矫治后牙宽度不调的临床疗效.方法:选择设计拔除磨牙矫治的7例骨性Ⅲ类错殆伴全牙弓反殆病例,男3例,女4例,年龄15~25岁,平均17.5岁,其中2例拔除下
孟河医派是明末清初源出江苏常州孟河的一大地域性医学流派,以费、马、巢、丁四大家族为主要代表.孟河医家临床经验丰富.学术造诣高深.其中在治咳方面,孟河医派四大家经过长
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7
为探究吕家坨井田地质构造格局,根据钻孔勘探资料,采用分形理论和趋势面分析方法,研究了井田7
本文对国外补充替代疗法医学期刊资源种类、分布情况、学术研究动态以及出版状况进行了调研,不仅为研究人员提供了最新的信息帮助,而且可进一步提高中国中医科学院作为中医药专业图书馆信息资源的利用率。