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甲型肝炎病毒8347毒株在人二倍体细胞（2BS株）中增殖4周，收获的病毒液经冻融、超声、过滤．德液用1：4000福尔马林、37℃灭活6天以上制成灭活疫苗，病毒灭括前的滴度（TCID50／ml）至少可达6．5左右．疫苗免疫豚鼠和小鼠三针后，75％～100％的动物甲肝抗体阳转。经超声处理后疫苗免疫原住可大幅度提高，三针后动物血清中的中和抗体效价可达1：20以上．
Hepatitis A virus 8347 strain was propagated in human diploid cells (2BS strain) for 4 weeks. The harvested virus solution was freeze-thawed, sonicated and filtered. German liquid with 1: 4000 formalin, inactivated at 37 ℃ for more than 6 days inactivated vaccine, the virus before titers (TCID50 / ml) at least up to 6.5 or so. After immunization of guinea pigs and mice with three doses of the vaccine, 75% -100% of the animals have positive anti-HBs. After the ultrasound treatment of vaccine immunogenic live can be greatly improved, after three-needle serum animal neutralization antibody titer of up to 1:20 or more.