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目的:制备和评价甘草次酸丙烯酸树脂E100纳米粒。方法:采用改良乳化-溶剂扩散法制备甘草次酸丙烯酸树脂E100纳米粒,通过考察载体与药物的比例、乳化剂的用量和搅拌速度等对纳米粒径、药物的包封率和载药量的影响,初步筛选处方。动态激光散射法测定粒径,透射电子显微镜观察外观形态。利用透析袋法,在含0.5%SDS的pH溶液中进行体外释放试验。结果:优化处方制备的纳米粒大小均匀,粒径约为(75.0±11.3)nm,包封率为(83.05±5.16)%,载药量为(29.22±1.60)%。甘草次酸的体外释放具有明显的pH依赖性。结论:改良乳化-溶剂扩散法制备了包封率高、大小均匀的pH依赖性甘草次酸纳米粒。
Objective: To prepare and evaluate glycyrrhetinic acid acrylic resin E100 nanoparticles. Methods: Glycyrrhetinic acid acrylic resin E100 nanoparticles were prepared by a modified emulsion-solvent diffusion method. The effects of the ratio of carrier to drug, the amount of emulsifier and stirring speed on the nanoparticles size, drug encapsulation efficiency and drug loading Impact, the initial screening of prescriptions. The particle size was measured by dynamic laser scattering method and the morphology was observed by transmission electron microscopy. In vitro release assay was carried out using a dialysis bag method in a 0.5% SDS-containing pH solution. Results: The size of nanoparticles prepared by optimized prescription was about 75.0 ± 11.3 nm with encapsulation efficiency of (83.05 ± 5.16)% and drug loading of (29.22 ± 1.60)%. Glycyrrhetinic acid release in vitro has obvious pH dependence. Conclusion: The pH-dependent glycyrrhetic acid nanoparticles with high encapsulation efficiency and uniform size were prepared by the modified emulsion-solvent diffusion method.