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目的建立膀胱癌T24耐药细胞株,并观察其相关生物学特性。方法采用丝裂霉素(MMC)浓度梯度持续递增法诱导建立膀胱癌T24耐药株,并从生长能力、克隆形成能力、迁移和侵袭能力、微球体形成能力等方面进行比较。结果膀胱癌T24耐药株与亲本细胞相比,其增殖速度明显高于亲本T24细胞;耐药株的克隆形成率、迁移和侵袭能力、成球能力均提高。结论低浓度丝裂霉素持续增量法可诱导形成膀胱癌T24耐药细胞株,且耐药株T24/MMC恶性程度更高,癌干细胞被富集。
Objective To establish bladder cancer T24 resistant cell lines and observe the related biological characteristics. Methods T24 drug resistant strains of bladder cancer were induced by increasing the concentration gradient of mitomycin C (MMC), and compared with growth ability, colony formation ability, migration and invasion ability, microsphere formation ability and so on. Results The proliferation rate of T24 drug-resistant strains of bladder cancer was significantly higher than that of the parental T24 cells. The clone formation rate, migration and invasion ability of the drug-resistant strains were all higher than those of the parental cells. CONCLUSION: Low dose MMC can induce the formation of bladder cancer T24 drug-resistant cell lines, and the drug-resistant T24 / MMC strains are more malignant and cancer stem cells are enriched.