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方法 将13名健康志愿者分成测试组8人(6男2女,年龄28～55岁),对照组5人(3男2女,年龄22～48岁)。研究前血细胞计数、血清铁蛋白浓度及常规临床参数均正常。测试组6人接受一次静脉注射重组红细胞生成素(rhEpo)300μg/kg,另2人150ug/kg。rhEpo静注后1周内每隔12小时抽取静脉血25ml,每次血样本均测红细胞计数、Hb浓度,用自动流式细胞仪测网织红细胞计数,并依其荧光强度分成高、中、低荧光网织红细胞,即HFR、MFR、LFR三类,且分别计其绝对值。为防止网织红细胞在体外成熟,抽取血样后立即检测。离心法制备血浆并置-70℃保存,用RIA测血浆Epo值,用免疫化学发光法测血清铁蛋白浓度,因该值个体差异较大,故每次结果均被正常化处理后以每例基础值的百分比来表示。
Methods Thirteen healthy volunteers were divided into test group (6 males and 2 females, aged 28-55 years) and control group (3 males and 2 females, aged 22-48 years). Pre-study blood cell counts, serum ferritin concentrations and routine clinical parameters were normal. Six patients in the test group received an intravenous injection of 300 μg / kg of rhEpo, and another two received 150 μg / kg of rhEpo. Venous blood 25ml was taken every 12 hours after intravenous injection of rhEpo within one week. The blood samples were measured for red blood cell count and Hb concentration. The reticulocyte count was measured by automatic flow cytometry and divided into high, Low-fluorescence reticulocytes, namely HFR, MFR, LFR three categories, respectively, and its absolute value. In order to prevent reticulocyte maturation in vitro, blood samples were taken immediately after testing. Plasma prepared by centrifugation and stored at -70 ℃, plasma Epo value was measured by RIA, immunochemiluminescence method for the determination of serum ferritin concentration, because the value of individual differences, so each time the results were normalized to each case The percentage of the base value.