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目的探讨内皮抑素和VEGF受体与慢性高原心脏病发病的关系以及红景天治疗高原心脏病的机理。方法建立慢性高原心脏病大鼠模型,分为对照组、吸烟运动刺激组、拟高原心脏病组和治疗组。利用免疫荧光组织化学和半定量RT-PCR方法检测内皮抑素和VEGF受体的表达水平。结果刺激组心肺组织与对照组相比,VEGF受体表达水平升高,而内皮抑素下降。治疗组与拟高原心脏病组相比VEGF受体表达水平降低,而内皮抑素升高。结论慢性高原心脏病发病与内皮细胞损伤密切相关,红景天可以减少内皮细胞损伤。
Objective To investigate the relationship between endostatin and VEGF receptors and the pathogenesis of chronic high altitude heart disease and the mechanism of Rhodiola treatment for high altitude heart disease. Methods A rat model of chronic high altitude heart disease was established and divided into control group, smoking motor stimulation group, plateau heart disease group and treatment group. The levels of endostatin and VEGF receptors were detected by immunofluorescence histochemistry and semi-quantitative RT-PCR. Results Compared with the control group, the expression of VEGF receptor in the cardiopulmonary tissue of the stimulating group increased, while endostatin decreased. Compared with the plateau heart disease group, the expression of VEGF receptor in the treatment group was decreased, and endostatin was increased. Conclusion The incidence of chronic altitude heart disease is closely related to the damage of endothelial cells. Rhodiola can reduce endothelial cell damage.