CFU-C曾用于研究白血病和正常细胞间关系。但其成灶细胞究竟由白血病细胞抑或由正常细胞而来,这无论在体内或用灶核型分析法均尚未确定。本文报告了一种用血浆凝块灶进行原位组织化学、核型分析的简单方法。依此法我们观察到RF小鼠白血病干细胞的成灶须有CSF参与,并查明了白血病细胞所产灶的形态和组化分析均与同系小鼠的正常干细胞相同。 CFU-C was used to study the relationship between leukemia and normal cells. However, whether or not the foci are established by leukemia cells or by normal cells has not been determined either in vivo or by nuclear karyotype analysis. This article reports a simple method of in situ histochemistry and karyotyping using plasma clots. According to this method, we observed that RF mouse leukemia stem cells have CSF involvement, and found that the morphology and histochemical analysis of leukemia cells were the same as that of normal mice.