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本文用荧光光谱法研究了皖南尖吻蝮蛇毒糖苷水解酶（ＮＡＤａｓｅ）的性质。在ｐＨ＜６时，ｐＨ对ＮＡＤａｓｅ的荧光强度影响较大，而在ｐＨ＞６，ｐＨ对ＮＡＤａｓｅ的荧光强度几乎没有什么影响，Ｃｕ２＋的加入可引起ＮＡＤａｓｅ的内源荧光强度的降低，通过荧光滴定测得Ｃｕ２＋与ＮＡＤａｓｅ结合常数ＫＥＭ为５．３×１０３（ｍｏｌ／Ｌ）－１。Ｉ－对ＮＡＤａｓｅ发光的淬灭作用很小，Ｉ－浓度为０．１ｍｏｌ／Ｌ和０．２ｍｏｌ／Ｌ时仅分别能淬灭为原来的１％和３％，但在１ｍｍｏｌ／ＬＥＤＴＡ存在情况下，Ｉ－对ＮＡＤａｓｅ发光的淬灭作用非常显著，Ｉ－浓度为０．１ｍｏｌ／Ｌ和０．２ｍｏｌ／Ｌ时淬灭分别为原来的２５％和４８％，因此Ｃｕ２＋对维持ＮＡＤａｓｅ结构起重要作用。
In this paper, the properties of Agkistrodon halysii glycoside hydrolase (NADase) from southern Anhui were studied by fluorescence spectroscopy. At pH <6, pH had a significant effect on the fluorescence intensity of NADase. At pH> 6, pH had little effect on the fluorescence intensity of NADase. The addition of Cu2 + caused the decrease of endogenous fluorescence intensity of NADase. The KEM of Cu2 + with NADase was 5.3 × 103 (mol / L) -1. The quenching effect of I- on NADase luminescence is very small. Only 1% and 3% of I- can be quenched with 0.1mol / L and 0.2mol / L, respectively. However, in the presence of 1mmol / , The quenching effect of I- on NADase luminescence is very significant. The quenching of I- at concentrations of 0.1 mol / L and 0.2 mol / L is 25% and 48% respectively, and Cu2 + plays an important role in maintaining NADase structure .