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目的：：建立测定丙烯酸树脂纳米粒中染料木素含量的 HPLC 方法。方法：色谱柱：DiamonsilTM C18柱(250 mm ×4.6 mm,5μm)；流动相：甲醇-水=85∶15(v∶v)；流速：1.0 ml·min-1；柱温：25℃；检测波长：261 nm；进样量：10μl。结果：药物峰与辅料峰分离度良好,染料木素浓度在1.0~100.0μg·ml-1范围内线性关系良好(r=0.9998),平均回收率为99.85%(RSD=0.65%,n=9)。结论：本方法简便,快捷,准确度高,专属性强,适用于测定染料木素丙烯酸树脂纳米粒的含量及包封率。“,”Objective:To establish an HPLC method for the determination of genistein in genistein-loaded Eudragit nanoparticles. Methods:The HPLC analysis was carried out on a DiamonsilTM C18(250 mm ×4.6 mm,5μm)column with the mobile phase of meth-anol-water (85∶15) at the flow rate of 1. 0 ml·min-1 . The detection wavelength was set at 261nm and the column temperature was 25℃. Results: A good separation was displayed between the drug peak and the excipient peaks. An excellent linearity range for genistein was obtained from 1.0 μg·ml-1 to 100.0 μg·ml-1(r =0.999 8). The recovery of genistein was 99.85%(RSD =0. 65%,n=9). Conclusion:The method is simple, rapid, accurate and specific, which is suitable for the determination of content and encapsulation efficiency of genistein-loaded nanoparticles.