论文部分内容阅读
目的探讨P/Q型钙离子通道拮抗剂ω-agatoxin-IVA(蜘蛛毒提取物)在猴脑缺血模型中的神经保护作用。方法成年广西猕猴8只,随机分成治疗组和对照组,每组4只。术前CT平扫和血管造影排除脑血管及颅内病变。各组动物经介入法插管至大脑中动脉,注入白体血栓造成大脑中动脉血栓栓塞,数字减影血管造影(DSA)证明栓塞成功,于术后30 min椎管内注射ω-agatoxin-IVA溶液,术后60 min行CT灌注扫描(CTP)观察梗死范围,术后1、15和30 d观察行为学表现,术后第30天行MRI扫描测量梗死体积,处死动物并取脑组织行HE染色,观察病理改变。结果术后介入血管造影和CTP均证明栓塞成功,术后60 min两组动物脑缺血范围在CTP图像上差异无统计学意义(P>0.05)。术后15和30 d行为学评分,治疗组分别为27.0±3.0和27.0±3.0,明显高于对照组的16.5±4.7和17.5±4.1,两组间差异有统计学意义(P<0.05)。术后30 d MRI扫描测量的梗死范围,治疗组明显低于对照组,两组间差异有统计学意义(64±38比193±24,P<0.05)。结论ω-agatoxin-IVA可以降低猴脑缺血后的梗死范围,减少神经功能损伤。
Objective To investigate the neuroprotective effect of P-Q-type calcium channel antagonist ω-agatoxin-IVA (arachnoid extract) in monkey model of cerebral ischemia. Methods Eight adult Guangxi macaques were randomly divided into treatment group and control group with 4 rats in each group. Preoperative CT scan and angiography exclude cerebrovascular and intracranial lesions. Animals in each group were intubated to the middle cerebral artery by interventional method, thromboembolism caused by white body thrombus caused by middle cerebral artery occlusion, digital angiography (DSA) proved successful in embolization, 30 min after spinal injection of omega-agatoxin-IVA The infarct area was observed by CT perfusion scanning (CTP) at 60 min after operation. The behavioral findings were observed on the 1st, 15th and 30th postoperative day. The infarct volume was measured by MRI on the 30th postoperative day. The animals were sacrificed and the brain tissue was subjected to HE Stain, observe the pathological changes. Results Postoperative interventional angiography and CTP demonstrated successful embolization. There was no significant difference in cerebral ischemic range between the two groups at 60 min after CTP (P> 0.05). Behavioral scores at 15 and 30 days after operation were 27.0 ± 3.0 and 27.0 ± 3.0 in the treatment group, which were significantly higher than those in the control group (16.5 ± 4.7 and 17.5 ± 4.1, respectively). There was significant difference between the two groups (P <0.05). The extent of infarction measured by MRI at 30 days after operation was significantly lower in the treatment group than in the control group, with a significant difference between the two groups (64 ± 38 vs. 193 ± 24, P <0.05). Conclusion Omega-agatoxin-IVA can reduce the infarction area after cerebral ischemia and reduce the neurological damage.