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目的:建立白头翁属5种药用植物皂苷类成分的HPLC指纹特征图谱,为这5种药用植物的鉴定及亲缘关系评价提供依据。方法:采用HPLC测定白头翁属药用植物的指纹图谱,依据获得的色谱数据对各样品进行分析与鉴定。结果:建立了白头翁属5种药用植物中皂苷类成分的HPLC指纹图谱,这5种药用植物均显示了其共性和各自的指纹特征;确立10个色谱峰为共有峰。运用系统聚类将14份样品聚成4类:第Ⅰ,Ⅱ,Ⅳ类分别为朝鲜白头翁,蒙古白头翁和白头翁;第Ⅲ类由兴安白头翁、细叶白头翁组成。朝鲜白头翁与兴安白头翁,蒙古白头翁与细叶白头翁之间的化学亲缘关系较远。结论:聚类结果与传统分类结果有较好的一致性。HPLC化学图谱可用于白头翁属植物的分类和鉴别研究,并为白头翁属植物指纹图谱研究和质量控制提供重要参考。
OBJECTIVE: To establish HPLC fingerprints of saponins from five medicinal plants in Pulsatilla, providing the basis for the identification and genetic relationship evaluation of the five medicinal plants. Methods: The fingerprint of Pulsatilla was determined by HPLC, and the samples were analyzed and identified according to the obtained chromatographic data. Results: HPLC fingerprints of saponins in five medicinal plants of Pulsatilla were established. All five medicinal plants showed their commonness and their fingerprints. The 10 peaks were identified as common peaks. Fourteen samples were grouped into four categories using cluster analysis: the first, the second and the fourth were Pulsatilla chinensis, Pulsatilla and Pulsatilla respectively; the third group consisted of Xingan Pulsatilla and Pulsatilla. North Korea Pulsatilla and Xingan Pulsatilla, Mongolian Pulsatilla and Pulsatilla chemical genetic relationship between the farther. Conclusion: The clustering results are in good agreement with the traditional classification results. HPLC chemical profiling can be used for the classification and identification of Pulsatilla plants, and provides an important reference for the fingerprinting and quality control of Pulsatilla.