论文部分内容阅读
目的了解血管紧张素Ⅱ对良性前列腺增生(BPH)大鼠前列腺细胞增殖和凋亡的作用。方法成年雄性Wistar大鼠随机分为正常组(A组),BPH模型组(B组)和BPH+血管紧张素转化酶抑制剂依那普利组(C组),每组10只。B组在双侧睾丸切除后1周开始皮下注射丙酸睾酮并灌饲生理盐水,C组在双侧睾丸切除术后1周开始皮下注射丙酸睾酮并灌饲依那普利。4周后观察各组大鼠前列腺重量和组织学变化,Ki-67免疫组化染色及TUNEL染色测定前列腺上皮和间质细胞的增殖和凋亡指数。结果3组大鼠前列腺指数[前列腺湿重(mg)／体重(g)]分别为1．54±0．09,1．65±0．07和1．59±0．09,B组大鼠前列腺指数显著大于A组和C组(P<0．05)。光镜显示B组前列腺上皮细胞增生明显,C组上皮细胞明显萎缩。A组上皮和间质细胞增殖率分别为(1．4±0．4)％和(1．1±0．4)％,B组为(3．0±0．4)％和(1．4±0．4)％,C组为(2．1±0．5)％和(1．2±0．5)％,B组上皮细胞增殖率显著高于A组和C组(P<0．05),而3组间质细胞的增殖率比较差异无统计学意义(P>0．05);3组上皮细胞凋亡率分别为(1．2±0．5)％,(1．1±0．3)％和(1．1±0．5)％(P>0．05)。结论血管紧张素Ⅱ在大鼠BPH组织中表达上调。血管紧张素Ⅱ可能影响大鼠前列腺细胞的增殖,但对细胞凋亡影响不明显。
Objective To investigate the effect of angiotensin Ⅱ on the proliferation and apoptosis of prostatic cells in benign prostatic hyperplasia (BPH) rats. Methods Adult male Wistar rats were randomly divided into normal group (group A), BPH model group (group B) and BPH + angiotensin converting enzyme inhibitor enalapril group (group C). Group B received testosterone propionate subcutaneously 1 week after bilateral orchiectomy and group C received testosterone propionate subcutaneously 1 week after bilateral orchiectomy and enalapril was infused. After 4 weeks, the weight and histological changes of prostate in rats were observed. Proliferation and apoptosis index of prostatic epithelium and stromal cells were measured by Ki-67 immunohistochemistry and TUNEL staining. Results The prostate index (wet weight of the prostate / weight) in the three groups were 1.54 ± 0.09, 1.65 ± 0.07 and 1.59 ± 0.09, respectively. The rats in group B Prostate index was significantly greater than the A and C groups (P <0.05). Light microscope showed that the proliferation of prostate epithelial cells in group B was obvious, and the epithelial cells in group C were obviously atrophied. The proliferation rates of epithelial and interstitial cells in group A were (1.4 ± 0.4)% and (1.1 ± 0.4)%, respectively, in group B were (3.0 ± 0.4)% and 4 ± 0.4% in group C, and (2.1 ± 0.5)% and (1.2 ± 0.5)% in group C. The proliferation rate of epithelial cells in group B was significantly higher than that in group A and C (P < 0.05). The proliferation rates of the three groups were not significantly different (P> 0.05). The apoptosis rates of the three groups were (1.2 ± 0.5)%, (1) .1 ± 0.3% and (1.1 ± 0.5)%, respectively (P> 0.05). Conclusion Angiotensin Ⅱ is up-regulated in BPH tissues of rats. Angiotensin Ⅱ may affect the proliferation of rat prostate cells, but the impact of apoptosis is not obvious.